Enhanced dynamics in the anomalous melting regime of DMPG lipid membranes

• Published in: Structural dynamics (Melville, N.Y.) N.Y.), 2020-09, Vol.7 (5), p.054704-054704
• Main Authors: Kelley, Elizabeth G., Nagao, Michihiro, Butler, Paul D., Porcar, Lionel, Farago, Bela
• Format: Article
• Language: English
• Subjects: Cooling; Dynamic structural analysis; Dynamics; Lipids; Melting; Membrane structures; Membranes; Phase transitions; Software packages; Spectrum analysis; Temperature
• ISSN: 2329-7778; 2329-7778
• DOI: 10.1063/4.0000031

Like many soft materials, lipids undergo a melting transition associated with a significant increase in their dynamics. At temperatures below the main melting transition (Tm), all molecular and collective dynamics are suppressed, while above Tm the alkyl tail motions, lipid diffusivity, and collective membrane undulations are at least an order of magnitude faster. Here we study the collective dynamics of dimyristoylphosphatidylglycerol (DMPG, di 14:0 PG) using neutron spin echo spectroscopy throughout its anomalous phase transition that occurs over a 12 °C–20° C wide temperature window. Our results reveal that the membranes are softer and more dynamic during the phase transition than at higher temperatures corresponding to the fluid phase and provide direct experimental evidence for the predicted increase in membrane fluctuations during lipid melting. These results provide new insights into the nanoscale lipid membrane dynamics during the melting transition and demonstrate how these dynamics are coupled to changes in the membrane structure.