Molecular cloning and expression characterization of flavonol synthase genes in peanut (Arachis hypogaea)

Flavonol is an important functional bioactive substance in peanut seeds, and plays important roles responding to abiotic stress. The flavonol content is closely related to the activity and regulation of gene expression patterns of flavonol synthase (FLS). In this study, eight FLS genes, AhFLS s were...

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Bibliographic Details
Published in:Scientific reports 2020-10, Vol.10 (1), p.17717-17717, Article 17717
Main Authors: Hou, Mingyu, Zhang, Yongjiang, Mu, Guojun, Cui, Shunli, Yang, Xinlei, Liu, Lifeng
Format: Article
Language:English
Subjects:
631/443
631/449
Arachis - enzymology
Arachis - metabolism
Arachis - physiology
Cloning, Molecular
Flavonoids - metabolism
Gene Expression
Genes, Plant
Humanities and Social Sciences
multidisciplinary
Oxidoreductases - genetics
Phylogeny
Plant Proteins - genetics
Science
Science (multidisciplinary)
Seedlings - metabolism
Stress, Physiological
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Summary:Flavonol is an important functional bioactive substance in peanut seeds, and plays important roles responding to abiotic stress. The flavonol content is closely related to the activity and regulation of gene expression patterns of flavonol synthase (FLS). In this study, eight FLS genes, AhFLS s were cloned and their expression characterization in different peanut organ and seedling under different abiotic stress were conducted. The results showed that the expressions levels of AhFLSs were differed in all assayed peanut organs and seedlings under abiotic stress treatments. Expression levels of AhFLS2 , AhFLS3 , AhFLS4 , and AhFLS6 were higher than those of other AhFLSs . The flavonol contents of peanut organs and seedlings under different abiotic stress were also determined using high performance liquid chromatography (HPLC). Dried mature peanut seeds were the organ tissue with the highest flavonol content, and flavonol content increased with seed development. Under abiotic stress treatments, the types of flavonols induced differed among stress treatments. Correlation analysis results suggested that eight AhFLS genes may have different functions in peanut. Moreover, changes in the expression of the eight genes appear to has substrate preference. These results can lay the foundation for the study of improving nutritional value of peanut seed and resistance of peanut plant.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-74763-w