Lupinus albus γ-Conglutin, a Protein Structurally Related to GH12 Xyloglucan-Specific Endo-Glucanase Inhibitor Proteins (XEGIPs), Shows Inhibitory Activity against GH2 β-Mannosidase

• Published in: International journal of molecular sciences 2020-10, Vol.21 (19), p.7305
• Main Authors: Benedetti, Stefano De, Galanti, Elisabetta, Capraro, Jessica, Magni, Chiara, Scarafoni, Alessio
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence - genetics; Amino acids; Cell walls; Endosperm; Enzymes; Glucans - chemistry; Glucans - genetics; Glycosidases; Glycoside hydrolase; Glycoside Hydrolases - antagonists & inhibitors; Glycoside Hydrolases - genetics; Inhibitors; Legumes; Lupinus - chemistry; Lupinus albus; Mannosidase; Microorganisms; Plant Proteins - genetics; Plant Proteins - ultrastructure; Proteins; Seed Storage Proteins - genetics; Seed Storage Proteins - ultrastructure; Seeds; Seeds - chemistry; Seeds - growth & development; Stoichiometry; Triticum - chemistry; Xylans - chemistry; Xylans - genetics; Xyloglucan
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21197305

γ-conglutin (γC) is a major protein of seeds, but its function is still unknown. It shares high structural similarity with xyloglucan-specific endo-glucanase inhibitor proteins (XEGIPs) and, to a lesser extent, with endoxylanase inhibitors (TAXI-I), active against fungal glycoside hydrolases GH12 and GH11, respectively. However, γC lacks both these inhibitory activities. Since β-galactomannans are major components of the cell walls of endosperm in several legume plants, we tested the inhibitory activity of γC against a GH2 β-mannosidase (EC 3.2.1.25). γC was actually able to inhibit the enzyme, and this effect was enhanced by the presence of zinc ions. The stoichiometry of the γC/enzyme interaction was 1:1, and the calculated was 1.55 μM. To obtain further insights into the interaction between γC and β-mannosidase, an in silico structural bioinformatic approach was followed, including some docking analyses. By and large, this work describes experimental findings that highlight new scenarios for understanding the natural role of γC. Although structural predictions can leave space for speculative interpretations, the full complexity of the data reported in this work allows one to hypothesize mechanisms of action for the basis of inhibition. At least two mechanisms seem plausible, both involving lupin-γC-peculiar structures.