An intrinsically disordered motif regulates the interaction between the p47 adaptor and the p97 AAA+ ATPase

VCP/p97, an enzyme critical to proteostasis, is regulated through interactions with protein adaptors targeting it to specific cellular tasks. One such adaptor, p47, forms a complex with p97 to direct lipid membrane remodeling. Here, we use NMR and other biophysical methods to study the structural dy...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2020-10, Vol.117 (42), p.26226-26236
Main Authors: Conicella, Alexander E., Huang, Rui, Ripstein, Zev A., Nguyen, Ai, Wang, Eric, Löhr, Thomas, Schuck, Peter, Vendruscolo, Michele, Rubinstein, John L., Kay, Lewis E.
Format: Article
Language:English
Subjects:
Adapters
Adaptor Proteins, Signal Transducing - chemistry
Adaptor Proteins, Signal Transducing - metabolism
Adenosine
Adenosine diphosphate
Adenosine Diphosphate - metabolism
Adenosine triphosphatase
Adenosine Triphosphatases - chemistry
Adenosine Triphosphatases - metabolism
Adenosine triphosphate
Adenosine Triphosphate - metabolism
Amino Acid Motifs
ATP
Biological Sciences
Dynamic structural analysis
Humans
Intrinsically Disordered Proteins - chemistry
Intrinsically Disordered Proteins - metabolism
Lipids
Models, Molecular
Molecular dynamics
NMR
Nuclear magnetic resonance
Nuclear Proteins - chemistry
Nuclear Proteins - metabolism
Nucleotides
Protein Binding
Protein Conformation
Task complexity
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Summary:VCP/p97, an enzyme critical to proteostasis, is regulated through interactions with protein adaptors targeting it to specific cellular tasks. One such adaptor, p47, forms a complex with p97 to direct lipid membrane remodeling. Here, we use NMR and other biophysical methods to study the structural dynamics of p47 and p47–p97 complexes. Disordered regions in p47 are shown to be critical in directing intra-p47 and p47–p97 interactions via a pair of previously unidentified linear motifs. One of these, an SHP domain, regulates p47 binding to p97 in a manner that depends on the nucleotide state of p97. NMR and electron cryomicroscopy data have been used as restraints in molecular dynamics trajectories to develop structural ensembles for p47–p97 complexes in adenosine diphosphate (ADP)- and adenosine triphosphate (ATP)-bound conformations, highlighting differences in interactions in the two states. Our study establishes the importance of intrinsically disordered regions in p47 for the formation of functional p47–p97 complexes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2013920117