Effects of naringin on reversing cisplatin resistance and the Wnt/β-catenin pathway in human ovarian cancer SKOV3/CDDP cells

Objective Ovarian cancer is one of three malignant tumors of the female reproductive system. Our previous studies showed that the traditional Chinese medicine naringin significantly inhibited the proliferation of platinum-resistant ovarian cancer cells in vitro, and that the mechanism may be related...

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Published in:Journal of international medical research 2020-10, Vol.48 (10), p.300060519887869-300060519887869
Main Authors: Zhu, Hong, Zou, Xia, Lin, ShiXin, Hu, Xin, Gao, Jun
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis
Cell growth
Cell Line, Tumor
Cell Proliferation
Chemotherapy
Cisplatin - pharmacology
Drug Resistance, Neoplasm
Female
Flavanones - pharmacology
Humans
Ovarian cancer
Ovarian Neoplasms - drug therapy
Pre-Clinical Research Report
Proteins
Signal transduction
Wnt Signaling Pathway
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Summary:Objective Ovarian cancer is one of three malignant tumors of the female reproductive system. Our previous studies showed that the traditional Chinese medicine naringin significantly inhibited the proliferation of platinum-resistant ovarian cancer cells in vitro, and that the mechanism may be related to the NF-κB pathway. Methods The MTT assay was used to detect the sensitivity of SKOV3 and SKOV3/CDDP cells to cisplatin, the effect of different naringin concentrations on the proliferation of SKOV3/CDDP cells, and the reversal of cisplatin resistance in naringin-treated SKOV3/CDDP cells. Western blotting was used to detect β-catenin, c-Myc, and cyclin D1 protein levels in the different cell lines. Results MTT results showed that different concentrations of naringin inhibited the proliferation of SKOV3 and SKOV3/CDDP cells, and that the inhibition increased with increasing concentrations and prolonged incubation times. Western blotting revealed that compared with controls (SKOV3/CDDP-0), β-catenin, c-Myc and cyclin D1 proteins levels were significantly decreased in SKOV3/CDDP-C, SKOV3/CDDP-N 20, and SKOV3/CDDP-CN 20 cells, suggesting that naringin inhibited the proliferation of SKOV3/CDDP cells in a concentration and time dependent manner. Conclusions Non-cytotoxic naringin reduced the expression of β-catenin, c-Myc, and cyclin D1 in SKOV3/CDDP cells and partially reversed cisplatin resistance in SKOV3/CDDP CN 20 cells.
ISSN:0300-0605
1473-2300
DOI:10.1177/0300060519887869