Expression of SASH1 in Preeclampsia and Its Effects on Human Trophoblast

Aim. To explore the involvement of SASH1 in preeclampsia. Methods. Expression of SASH1 was determined by qPCR, WB, and immunohistochemistry in the placenta of both normal and preeclamptic pregnancies. The SASH1 gene of human HTR-8/SVneo cells was overexpressed by transfection of pEZ-Lv206-SASH1. Aft...

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Bibliographic Details
Published in:BioMed research international 2020, Vol.2020 (2020), p.1-8
Main Authors: Yu, Yanhong, Huang, Liping, Jiang, Sijia, Liu, Shisan
Format: Article
Language:English
Subjects:
Adapter proteins
Adult
Antibodies
Apoptosis
Apoptosis - genetics
Assaying
Biopsy
Biotechnology
Cancer
Cell adhesion & migration
Cell Line
Cell migration
Cell Movement - genetics
Cell proliferation
Cell Proliferation - genetics
Cholecystokinin
Development and progression
Female
Flow cytometry
Gene expression
Genetic aspects
Gestational age
Health aspects
Humans
Hypertension
Immunohistochemistry
Obstetrical research
Placenta
Placenta - metabolism
Pre-eclampsia
Pre-Eclampsia - genetics
Pre-Eclampsia - metabolism
Preeclampsia
Pregnancy
Risk factors
Software
Statistical analysis
Transfection
Trophoblast
Trophoblasts - metabolism
Tumor Suppressor Proteins - genetics
Tumor Suppressor Proteins - metabolism
Up-Regulation - genetics
Womens health
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Summary:Aim. To explore the involvement of SASH1 in preeclampsia. Methods. Expression of SASH1 was determined by qPCR, WB, and immunohistochemistry in the placenta of both normal and preeclamptic pregnancies. The SASH1 gene of human HTR-8/SVneo cells was overexpressed by transfection of pEZ-Lv206-SASH1. After that, the CCK-8 assay, EdU assay, transwell assay, and flow cytometry were used to examine the cell proliferation, migration, invasion, and apoptosis. Results. Higher expression of SASH1 was detected in placental tissues collected from patients with preeclampsia, compared with those from gestational age-matched control samples. The expression of SASH1 was significantly enhanced by transfection with pEZ-Lv206-SASH1 in HTR-8/SVneo cells. In addition, the HTR-8/SVneo cells transfected with pEZ-Lv206-SASH1 exhibited significantly reduced proliferation, migration, and invasion ability compared to the cells in the empty vector group and normal group. Flow cytometry analysis demonstrated that the apoptosis rate of cells transfected with pEZ-Lv206-SASH1 was significantly higher than that of cells transfected with empty vector and untreated cells. Conclusions. SASH1 is significantly upregulated in the placenta of preeclampsia, and overexpression of SASH1 can inhibit the proliferation, migration, and invasion, but induce apoptosis of trophoblast cells in vitro.
ISSN:2314-6133
2314-6141
DOI:10.1155/2020/5058260