Contralateral spreading of substances following intratympanic nanoparticle-conjugated gentamicin injection in a rat model

This study was performed to investigate the Eustachian tube as a potential route for contralateral spreading following intratympanic nanoparticle (NP)-conjugated gentamicin injection in a rat model. Sprague–Dawley rats were divided into three groups and substances were injected in the right ear: gro...

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Bibliographic Details
Published in:Scientific reports 2020-10, Vol.10 (1), p.18636, Article 18636
Main Authors: Lee, Sang-Yeon, Kim, Jeonghyo, Oh, Sangjin, Jung, Gaon, Jeong, Ki-Jae, Tan Tran, Van, Hwang, Dajeong, Kim, SungIl, Song, Jae-Jin, Suh, Myung-Whan, Lee, Jaebeom, Koo, Ja-Won
Format: Article
Language:English
Subjects:
639/925
692/308
Animals
Cochlea
Computer applications
Drug delivery
Ear
Gentamicin
Gentamicins - administration & dosage
Hair cells
Humanities and Social Sciences
Injection
Injection, Intratympanic
Magnetic Resonance Imaging - methods
multidisciplinary
Nanoparticles
Nanoparticles - chemistry
Rats
Rats, Sprague-Dawley
Rodents
Science
Science (multidisciplinary)
Spreading
Tympanum
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Summary:This study was performed to investigate the Eustachian tube as a potential route for contralateral spreading following intratympanic nanoparticle (NP)-conjugated gentamicin injection in a rat model. Sprague–Dawley rats were divided into three groups and substances were injected in the right ear: group 1 (fluorescent magnetic nanoparticles [F-MNPs], n  = 4), group 2 (F-MNP-conjugated gentamicin [F-MNP@GM], n  = 2), and control group (no injections, n  = 2). T2-weighted sequences corresponding to the regions of interest at 1, 2, and 3 h after intratympanic injection were evaluated, along with immunostaining fluorescence of both side cochlea. The heterogeneous signal intensity of F-MNPs and F-MNP@GM on T2-weighted images, observed in the ipsilateral tympanum, was also detected in the contralateral tympanum in 4 out of 6 rats, recapitulating fluorescent nanoparticles in the contralateral cochlear hair cells. Computational simulations demonstrate the contralateral spreading of particles by gravity force following intratympanic injection in a rat model. The diffusion rate of the contralateral spreading relies on the sizes and surface charges of particles. Collectively, the Eustachian tube could be a route for contralateral spreading following intratympanic injection. Caution should be taken when using the contralateral ear as a control study investigating inner-ear drug delivery through the transtympanic approach.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-75725-y