A Novel Bioactive Endodontic Sealer Containing Surface-Reaction-Type Prereacted Glass-Ionomer Filler Induces Osteoblast Differentiation

Surface-reaction-type prereacted glass-ionomer (S-PRG) fillers exhibit bioactive properties by the release of multiple ions. This study examined whether a novel endodontic sealer containing S-PRG fillers (PRG+) has the capacity to induce osteoblast differentiation. Kusa-A1 osteoblastic cells were cu...

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Bibliographic Details
Published in:Materials 2020-10, Vol.13 (20), p.4477
Main Authors: Kawashima, Nobuyuki, Hashimoto, Kentaro, Kuramoto, Masashi, Bakhit, Alamuddin, Wakabayashi, Yasumiko, Okiji, Takashi
Format: Article
Language:English
Subjects:
Alizarin
Alkaline phosphatase
Biocompatibility
Biological activity
Biomedical materials
Calcium
Composite materials
Differentiation
Fillers
Fluorides
Genomics
Humidity
Immunofluorescence
Ionomers
Kinases
Membranes
Mineralization
Osteoblasts
Phosphatase
Phosphorylation
Polymerase chain reaction
Receptors
Sealing compounds
Silica
Silicon dioxide
Variance analysis
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Summary:Surface-reaction-type prereacted glass-ionomer (S-PRG) fillers exhibit bioactive properties by the release of multiple ions. This study examined whether a novel endodontic sealer containing S-PRG fillers (PRG+) has the capacity to induce osteoblast differentiation. Kusa-A1 osteoblastic cells were cultured with extracts of PRG+, PRG− (an experimental sealer containing S-PRG-free silica fillers), AH Plus (an epoxy-resin-based sealer), and Canals N (a zinc-oxide noneugenol sealer). Cell viability and mineralized nodule formation were determined using WST-8 assay and Alizarin red staining, respectively. Osteoblastic-marker expression was analyzed with RT-qPCR and immunofluorescence. Phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) was determined with Western blotting. Extracts of freshly mixed PRG+, PRG−, and AH Plus significantly decreased cell growth, but extracts of the set samples were not significantly cytotoxic. Set PRG+ significantly upregulated mRNAs for alkaline phosphatase and bone sialoprotein (IBSP) compared to set PRG−, and upregulation was blocked by NPS2143, a calcium-sensing receptor antagonist. Set PRG+ significantly accelerated IBSP expression, mineralized nodule formation, and enhanced the phosphorylation of ERK and p38 compared with set PRG−. In conclusion, PRG+ induced the differentiation and mineralization of Kusa-A1 cells via the calcium-sensing receptor-induced activation of ERK and p38 MAPK.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma13204477