Confocal Laser Endomicroscopy Assessment of Pituitary Tumor Microstructure: A Feasibility Study

This is the first study to assess confocal laser endomicroscopy (CLE) use within the transsphenoidal approach and show the feasibility of obtaining digital diagnostic biopsies of pituitary tumor tissue after intravenous fluorescein injection. We confirmed that the CLE probe reaches the tuberculum se...

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Bibliographic Details
Published in:Journal of clinical medicine 2020-09, Vol.9 (10), p.3146
Main Authors: Belykh, Evgenii, Ngo, Brandon, Farhadi, Dara S., Zhao, Xiaochun, Mooney, Michael A., White, William L., Daniels, Jessica K., Little, Andrew S., Eschbacher, Jennifer M., Preul, Mark C.
Format: Article
Language:English
Subjects:
Biopsy
Brain cancer
Clinical medicine
Decision making
Feasibility studies
Histology
Lasers
Pituitary gland
Scanners
Surgery
Tumors
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Summary:This is the first study to assess confocal laser endomicroscopy (CLE) use within the transsphenoidal approach and show the feasibility of obtaining digital diagnostic biopsies of pituitary tumor tissue after intravenous fluorescein injection. We confirmed that the CLE probe reaches the tuberculum sellae through the transnasal transsphenoidal corridor in cadaveric heads. Next, we confirmed that CLE provides images with identifiable histological features of pituitary adenoma. Biopsies from nine patients who underwent pituitary adenoma surgery were imaged ex vivo at various times after fluorescein injection and were assessed by a blinded board-certified neuropathologist. With frozen sections used as the standard, pituitary adenoma was diagnosed as “definitively” for 13 and as “favoring” in 3 of 16 specimens. CLE digital biopsies were diagnostic for pituitary adenoma in 10 of 16 specimens. The reasons for nondiagnostic CLE images were biopsy acquisition 10 min after fluorescein injection (n = 5) and blood artifacts (n = 1). In conclusion, fluorescein provided sufficient contrast for CLE at a dose of 2 mg/kg, optimally 1–10 min after injection. These results provide a basis for further in vivo studies using CLE in transsphenoidal surgery.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm9103146