Three-dimensional analysis of the heart function and effect cholinergic agonists in the cockroach Gromphadorhina portentosa

Many relevant aspects of mammal’s cardiac physiology have been mainly investigated in insect models such as Drosophila melanogaster and Periplaneta americana . Cardiac function has been poorly studied in the cockroach Gromphadorhina portentosa, which has some advantages for experimental purposes suc...

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Bibliographic Details
Published in:Journal of Comparative Physiology 2020-11, Vol.206 (6), p.857-870
Main Authors: Claros-Guzmán, Alfonso, Rodríguez, Martín G., Heredia-Rivera, Birmania, González-Segovia, Rodolfo
Format: Article
Language:English
Subjects:
Acetylcholine
Agonists
Animal Physiology
Biomedical and Life Sciences
Cholinergics
Cockroaches
Diastole
Digital imaging
Dimensional analysis
Gromphadorhina portentosa
Heart
Heart function
Heart valves
Hemolymph
Insects
Life Sciences
Mathematical analysis
Muscarine
Neurosciences
Neurotransmitters
Nicotine
Organs
Original Paper
Paralysis
Physiological responses
Physiology
Recording
Systole
Three dimensional analysis
Zoology
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Summary:Many relevant aspects of mammal’s cardiac physiology have been mainly investigated in insect models such as Drosophila melanogaster and Periplaneta americana . Cardiac function has been poorly studied in the cockroach Gromphadorhina portentosa, which has some advantages for experimental purposes such as an easier culture, bigger organs and a robust physiology. On the other hand, the study of cardiac physiology in insects has been largely improved since the arrival of digital imaging technologies for recording purposes. In the present work, we introduce a methodology of video recording coupled to an isotonic transducer for a three-dimensional analysis of the heart and intracardiac valves of G. portentosa . We used this methodology for assessing the physiological responses of the cockroach heart upon the application of different cholinergic neurotransmitters (acetylcholine, nicotine and muscarine). We recorded in detail the relationship between intracardiac valves movement, hemolymph flow, diastole and systole. Acetylcholine and nicotine induced a biphasic effect on the cardiac frequency. Acetylcholine increased the diastolic opening. Nicotine at high concentration caused paralysis. Muscarine induced no major effects. These findings suggest a combined action of cholinergic agonists for a finely tuned the cardiac frequency, intracardiac valves function and cardiac cycle.
ISSN:0340-7594
1432-1351
DOI:10.1007/s00359-020-01443-5