Unraveling the adult cell progeny of early postnatal progenitor cells

• Published in: Scientific reports 2020-11, Vol.10 (1), p.19058-19058, Article 19058
• Main Authors: Sánchez-González, Rebeca, Salvador, Nieves, López-Mascaraque, Laura
• Format: Article
• Language: English
• Subjects: 631/378/2571/2579; 631/378/368/2431; Age Factors; Aging; Animals; Astrocytes; Biomarkers; Cell cycle; Cell Differentiation; Cell Tracking; Clonal Evolution; Corpus callosum; Female; Gene Expression Regulation, Developmental; Genomes; Glial cells; Glial stem cells; Heterogeneity; Humanities and Social Sciences; Male; Mice; Molecular Imaging; multidisciplinary; Neural stem cells; Neural Stem Cells - cytology; Neural Stem Cells - metabolism; Neurogenesis; Neurons; Ontogeny; Plasmids; Progenitor cells; Proteins; Science; Science (multidisciplinary); Stem Cells - cytology; Stem Cells - metabolism; Temporal lobe
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-75973-y

NG2-glia, also referred to as oligodendrocyte precursor cells or polydendrocytes, represent a large pool of proliferative neural cells in the adult brain that lie outside of the two major adult neurogenic niches. Although their roles are not fully understood, we previously reported significant clonal expansion of adult NG2-cells from embryonic pallial progenitors using the StarTrack lineage-tracing tool. To define the contribution of early postnatal progenitors to the specific NG2-glia lineage, we used NG2-StarTrack. A temporal clonal analysis of single postnatal progenitor cells revealed the production of different glial cell types in distinct areas of the dorsal cortex but not neurons. Moreover, the dispersion and size of the different NG2 derived clonal cell clusters increased with age. Indeed, clonally-related NG2-glia were located throughout the corpus callosum and the deeper layers of the cortex. In summary, our data reveal that postnatally derived NG2-glia are proliferative cells that give rise to NG2-cells and astrocytes but not neurons. These progenitors undergo clonal cell expansion and dispersion throughout the adult dorsal cortex in a manner that was related to aging and cell identity, adding new information about the ontogeny of these cells. Thus, identification of clonally-related cells from specific progenitors is important to reveal the NG2-glia heterogeneity.