Light and ripening-regulated BBX protein-encoding genes in Solanum lycopersicum

• Published in: Scientific reports 2020-11, Vol.10 (1), p.19235-19235, Article 19235
• Main Authors: Lira, Bruno Silvestre, Oliveira, Maria José, Shiose, Lumi, Wu, Raquel Tsu Ay, Rosado, Daniele, Lupi, Alessandra Cavalcanti Duarte, Freschi, Luciano, Rossi, Magdalena
• Format: Article
• Language: English
• Subjects: 631/449/1736; 631/449/2686; Chloroplasts; Fruit - genetics; Fruit - metabolism; Fruits; Gene Expression Regulation, Plant - physiology; Genomes; Humanities and Social Sciences; Light; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; multidisciplinary; Multigene Family - physiology; Photomorphogenesis; Phylogeny; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant tissues; Proteins; Ripening; Science; Science (multidisciplinary); Seedlings; Tomatoes; Transcription Factors - biosynthesis; Transcription Factors - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-76131-0

Light controls several aspects of plant development through a complex signalling cascade. Several B-box domain containing proteins (BBX) were identified as regulators of Arabidopsis thaliana seedling photomorphogenesis. However, the knowledge about the role of this protein family in other physiological processes and species remains scarce. To fill this gap, here BBX protein encoding genes in tomato genome were characterised. The robust phylogeny obtained revealed how the domain diversity in this protein family evolved in Viridiplantae and allowed the precise identification of 31 tomato SlBBX proteins. The mRNA profiling in different organs revealed that SlBBX genes are regulated by light and their transcripts accumulation is directly affected by the chloroplast maturation status in both vegetative and fruit tissues. As tomato fruits develops, three SlBBXs were found to be upregulated in the early stages, controlled by the proper chloroplast differentiation and by the PHYTOCHROME (PHY)-dependent light perception. Upon ripening, other three SlBBXs were transcriptionally induced by RIPENING INHIBITOR master transcriptional factor, as well as by PHY-mediated signalling and proper plastid biogenesis. Altogether, the results obtained revealed a conserved role of SlBBX gene family in the light signalling cascade and identified putative members affecting tomato fruit development and ripening.