Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up...

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Published in:Medicina oral, patología oral y cirugía bucal patología oral y cirugía bucal, 2020-11, Vol.25 (6), p.e752-e761
Main Authors: Vieira, V, Campos, L-H, Jesus, L-H, Klabunde, C, Gamba, T-D, Flores, I-L, Oliveira, M-G, Rados, P-V
Format: Article
Language:English
Subjects:
Aldehyde Dehydrogenase 1 Family
Biomarkers, Tumor
Dentistry
Epithelial-Mesenchymal Transition
Head and Neck Neoplasms
Humans
Neoplasm Recurrence, Local
Squamous Cell Carcinoma of Head and Neck
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Summary:The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up over an 8-year period. For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical expression with different variables, considering p
ISSN:1698-6946
1698-4447
1698-6946
DOI:10.4317/medoral.23777