Evaluation of an automated immunoturbidimetric assay for detecting canine C-reactive protein

C-reactive protein (CRP) is a major acute-phase protein, and it is produced by the liver in response to a pro-inflammatory stimulus. Given that human and canine CRP have a similar molecular structure, the assays used for human CRP detection have been used to measure CRP concentrations in dogs. We ev...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2020-11, Vol.32 (6), p.948-952
Main Authors: Berlanda, Michele, Valente, Carlotta, Bonsembiante, Federico, Badon, Tamara, Bedin, Silvia, Contiero, Barbara, Guglielmini, Carlo, Poser, Helen
Format: Article
Language:English
Subjects:
Animals
Biological Assay - veterinary
Blood Chemical Analysis - methods
Blood Chemical Analysis - standards
Blood Chemical Analysis - veterinary
Brief Communications
C-Reactive Protein - analysis
Dog Diseases - blood
Dog Diseases - diagnosis
Dogs
Immunoturbidimetry - veterinary
Limit of Detection
Reference Values
Reproducibility of Results
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Summary:C-reactive protein (CRP) is a major acute-phase protein, and it is produced by the liver in response to a pro-inflammatory stimulus. Given that human and canine CRP have a similar molecular structure, the assays used for human CRP detection have been used to measure CRP concentrations in dogs. We evaluated the use of a human CRP assay (Biotecnica CRP assay) and validated its application in dogs. We analyzed 91 canine serum samples with a fully automated analyzer. Our validation was based on the evaluation of imprecision, limits of linearity, limits of quantification, and an evaluation of interferences. The new assay was also compared with the Randox CRP assay, a validated assay for the measurement of CRP. Intra- and inter-assay repeatability were
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638720960065