Genomic and transcriptomic variation defines the chromosome-scale assembly of Haemonchus contortus, a model gastrointestinal worm

• Published in: Communications biology 2020-11, Vol.3 (1), p.656-656, Article 656
• Main Authors: Doyle, Stephen R., Tracey, Alan, Laing, Roz, Holroyd, Nancy, Bartley, David, Bazant, Wojtek, Beasley, Helen, Beech, Robin, Britton, Collette, Brooks, Karen, Chaudhry, Umer, Maitland, Kirsty, Martinelli, Axel, Noonan, Jennifer D., Paulini, Michael, Quail, Michael A., Redman, Elizabeth, Rodgers, Faye H., Sallé, Guillaume, Shabbir, Muhammad Zubair, Sankaranarayanan, Geetha, Wit, Janneke, Howe, Kevin L., Sargison, Neil, Devaney, Eileen, Berriman, Matthew, Gilleard, John S., Cotton, James A.
• Format: Article
• Language: English
• Subjects: 38/39; 45/23; 45/91; 631/114/2785; 631/1647/2217/457; 631/1647/2217/748; 631/1647/334/2246; 64/11; 692/699/255/1715; Animal biology; Animals; Anthelmintic agents; Biology; Biomedical and Life Sciences; Caenorhabditis elegans - genetics; Chromosomes; Chromosomes - genetics; Conserved sequence; Drug resistance; Ecology, environment; Evolution; Female; Gene expression; Gene order; Gene regulation; Genetic diversity; Genetics; Genome, Helminth - genetics; Genomes; Genomics; Haemonchiasis - parasitology; Haemonchus - genetics; Haemonchus - metabolism; Haemonchus - physiology; Haemonchus contortus; Humans; Intestinal Diseases, Parasitic - parasitology; Life Cycle Stages - genetics; Life cycles; Life Sciences; Male; Models, Biological; Nucleotide sequence; Parasites; Pathogens; Symbiosis; Transcription; Transcriptome - genetics; Vaccine development; Veterinary medicine and animal Health
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-020-01377-3

Haemonchus contortus is a globally distributed and economically important gastrointestinal pathogen of small ruminants and has become a key nematode model for studying anthelmintic resistance and other parasite-specific traits among a wider group of parasites including major human pathogens. Here, we report using PacBio long-read and OpGen and 10X Genomics long-molecule methods to generate a highly contiguous 283.4 Mbp chromosome-scale genome assembly including a resolved sex chromosome for the MHco3(ISE).N1 isolate. We show a remarkable pattern of conservation of chromosome content with Caenorhabditis elegans , but almost no conservation of gene order. Short and long-read transcriptome sequencing allowed us to define coordinated transcriptional regulation throughout the parasite’s life cycle and refine our understanding of cis- and trans- splicing. Finally, we provide a comprehensive picture of chromosome-wide genetic diversity both within a single isolate and globally. These data provide a high-quality comparison for understanding the evolution and genomics of Caenorhabditis and other nematodes and extend the experimental tractability of this model parasitic nematode in understanding helminth biology, drug discovery and vaccine development, as well as important adaptive traits such as drug resistance. Stephen Doyle et al. report the chromosome-scale genome assembly and transcriptome sequence data of Haemonchus contortus , a key parasitic nematode model. These data show nearly complete conservation of chromosome content with C. elegans and brings insight into transcriptional regulation and chromosome-wide genetic diversity in this important pathogen.