Hospital Wastewater-Important Source of Multidrug Resistant Coliform Bacteria with ESBL-Production

This work compares the prevalence of antibiotic resistant coliform bacteria in hospital wastewater effluents in Slovak (SR) and Czech Republic (ČR). It also describes selected antibiotic resistant isolates in view of resistance mechanism and virulence factor. The highest number of multidrug resistan...

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Bibliographic Details
Published in:International journal of environmental research and public health 2020-10, Vol.17 (21), p.7827
Main Authors: Lépesová, Kristína, Olejníková, Petra, Mackuľak, Tomáš, Cverenkárová, Klára, Krahulcová, Monika, Bírošová, Lucia
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Bacteria
beta-Lactamases - genetics
Biofilms
Coliforms
Czech Republic - epidemiology
Drug Resistance, Multiple, Bacterial - drug effects
Drug Resistance, Multiple, Bacterial - genetics
E coli
Effluents
Efflux
Genes
Health care
Health facilities
Hospital wastes
Hospitals
Identification
Laboratories
Medical wastes
Microbial Sensitivity Tests
Multidrug resistance
Multidrug resistant organisms
Phenotypes
Resistance factors
Sewage
Sewer systems
Sewerage
Virulence
Waste Water
Wastewater treatment
Wastewater treatment plants
β Lactamase
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Summary:This work compares the prevalence of antibiotic resistant coliform bacteria in hospital wastewater effluents in Slovak (SR) and Czech Republic (ČR). It also describes selected antibiotic resistant isolates in view of resistance mechanism and virulence factor. The highest number of multidrug resistant bacteria was detected in samples from the hospital in Valašské Meziříčí (ČR). More than half of resistant isolates showed multidrug resistance phenotype as well as strong ability to form biofilm. In 42% of isolates efflux pump overproduction was detected together with and genes. The production of extended-spectrum β-lactamases in coliform isolates was encoded mainly by , and genes. About 62% of resistants contained a combination of two or more extended spectrum beta-lactamases (ESBL) genes. Our results strengthen the fact that hospital effluents are a source of multidrug resistant bacteria which can spread their resistance genes to other bacteria in wastewater treatment plants (WWTPs). Accordingly, hospital wastewater should be better treated before it enters urban sewerage.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph17217827