Metabolomics profiling of plasma, urine and saliva after short term training in young professional football players in Saudi Arabia

Metabolomics profiling was carried out to observe the effect of short-term intensive physical activity on the metabolome of young Saudi professional football players. Urine, plasma and saliva were collected on 2 days pre- and post-training. An Orbitrap Exactive mass spectrometer was used to analyze...

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Bibliographic Details
Published in:Scientific reports 2020-11, Vol.10 (1), p.19759, Article 19759
Main Authors: Alzharani, Mansour A., Alshuwaier, Ghareeb O., Aljaloud, Khalid S., Al-Tannak, Naser F., Watson, David G.
Format: Article
Language:English
Subjects:
631/45
631/45/320
Adult
Athletes - statistics & numerical data
Athletic Performance - physiology
Carnitine
Chromatography
Exercise
Football
Humanities and Social Sciences
Humans
Male
Metabolites
Metabolome
Metabolomics
multidisciplinary
Physical activity
Physical fitness
Physical training
Plasma
Plasma - metabolism
Principal components analysis
Professional football
Saliva
Saliva - metabolism
Saudi Arabia
Science
Science (multidisciplinary)
Training
Urinalysis - methods
Urine
Young Adult
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Summary:Metabolomics profiling was carried out to observe the effect of short-term intensive physical activity on the metabolome of young Saudi professional football players. Urine, plasma and saliva were collected on 2 days pre- and post-training. An Orbitrap Exactive mass spectrometer was used to analyze the samples. A reversed-phase (RP) column was used for the analysis of non-polar plasma metabolites, and a ZIC-pHILIC column was used for the analysis of plasma, saliva and urine. mzMine was used to extract the data, and the results were modelled using Simca-P 14.1 software. There was no marked variation in the metabolite profiles between pre day 1 and 2 or between post day 1 and 2 according to principal components analysis (PCA). When orthogonal partial least squares (OPLSDA) modelling was also used, and then models could be fitted based on a total number of metabolites of 75, 16 and 32 for urine, plasma and saliva using hydrophilic interaction chromatography (HILIC) and 6 for analysis of plasma with reversed-phase (RP) chromatography respectively. The present study concludes that acylcarnitine may increase post-exercise in football players suggesting that they may burn fat rather than glucose. The levels of carnitine metabolites in plasma post-exercise could provide an important indicator of fitness.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-75755-6