Evaluation of Anti-oxidant and Anti-biofilm Activities of Biogenic Surfactants Derived from Bacillus amyloliquefaciens and Pseudomonas aeruginosa

• Published in: Iranian journal of pharmaceutical research : IJPR 2020-01, Vol.19 (2), p.115-126
• Main Authors: Abdollahi, Soosan, Tofighi, Zahra, Babaee, Tahereh, Shamsi, Mohammad, Rahimzadeh, Ghazal, Rezvanifar, Hossein, Saeidi, Elaheh, Mohajeri Amiri, Morteza, Saffari Ashtiani, Yasaman, Samadi, Nasrin
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 1735-0328; 1726-6890
• DOI: 10.22037/IJPR.2020.1101033

Biosurfactants, the microbial originated surface active agents, can modify the physicochemical properties of surfaces and reduce the bacterial adhesion via changing bacterial adhesion interactions on surfaces. They were also able to block oxidative chain reactions and might show antioxidant properties. The goal of this study was to evaluate the antioxidant and antibiofilm activities of biosurfactants which were derived from two autochthonous biosurfactant-producing strains, Bacillus amyloliquefaciens NS6 (surfactin), and Pseudomonas aeruginosa MN1 (rhamnolipids). Their antioxidant activities were determined by ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. Ferric thiocyanate (FTC) assay was used for determination of their lipid peroxidation inhibition capacity. Their effect to reduce the adhesion of Streptococcus mutans on polystyrene surfaces and disruption of its pre-formed biofilms were also investigated. Our results indicated that surfactin showed higher antioxidant activity than rhamnolipids and showed relatively similar efficiency to BHA that suggests it as a good alternative for synthetic antioxidants. In other hand, rhamnolipid conditioned surfaces showed higher antiadhesive and antibiofilm activity in comparison with surfactin treated surfaces.