Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

This study aims to utilize the cell-biomass (CB) and supernatant (CFS) of zinc-tolerant Lactobacillus plantarum TA4 as a prospective nanofactory to synthesize ZnO NPs. The surface plasmon resonance for the biosynthesized ZnO NPs-CFS and ZnO NPs-CB was 349 nm and 351 nm, respectively, thereby confirm...

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Bibliographic Details
Published in:Scientific reports 2020-11, Vol.10 (1), p.19996, Article 19996
Main Authors: Mohd Yusof, Hidayat, Abdul Rahman, Nor’Aini, Mohamad, Rosfarizan, Zaidan, Uswatun Hasanah, Samsudin, Anjas Asmara
Format: Article
Language:English
Subjects:
631/1647
631/326
631/553
631/57
631/61
639/301
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Biocompatibility
Biocompatible Materials - chemistry
Biomass
Biosynthesis
Cell Line
Chlorocebus aethiops
Humanities and Social Sciences
Lactobacillus plantarum
Lactobacillus plantarum - metabolism
Metal Nanoparticles - administration & dosage
Metal Nanoparticles - chemistry
Microbial Sensitivity Tests - methods
multidisciplinary
Nanoparticles
Particle Size
Prospective Studies
Science
Science (multidisciplinary)
Surface plasmon resonance
Vero Cells
Zinc oxide
Zinc Oxide - chemistry
Zinc oxides
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Summary:This study aims to utilize the cell-biomass (CB) and supernatant (CFS) of zinc-tolerant Lactobacillus plantarum TA4 as a prospective nanofactory to synthesize ZnO NPs. The surface plasmon resonance for the biosynthesized ZnO NPs-CFS and ZnO NPs-CB was 349 nm and 351 nm, respectively, thereby confirming the formation of ZnO NPs. The FTIR analysis revealed the presence of proteins, carboxyl, and hydroxyl groups on the surfaces of both the biosynthesized ZnO NPs that act as reducing and stabilizing agents. The DLS analysis revealed that the poly-dispersity indexes was less than 0.4 for both ZnO NPs. In addition, the HR-TEM micrographs of the biosynthesized ZnO NPs revealed a flower-like pattern for ZnO NPs-CFS and an irregular shape for ZnO NPs-CB with particles size of 291.1 and 191.8 nm, respectively. In this study, the biosynthesized ZnO NPs exhibited antibacterial activity against pathogenic bacteria in a concentration-dependent manner and showed biocompatibility with the Vero cell line at specific concentrations. Overall, CFS and CB of L. plantarum TA4 can potentially be used as a nanofactory for the biological synthesis of ZnO NPs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-76402-w