Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus

In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is sc...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports 2020-12, Vol.47 (12), p.9959-9965
Main Authors: González Altamiranda, Erika A., Arias, María E., Kaiser, Germán G., Mucci, Nicolás C., Odeón, Anselmo C., Felmer, Ricardo N.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Blastocysts
Cell culture
Culture media
Diarrhea
Embryogenesis
Embryos
Experimental infection
Gametes
Gene expression
Histology
Immune response
In vitro fertilization
Inflammation
Life Sciences
Livestock
Morphology
Short Communication
Titration
TLR7 protein
Toll-like receptors
Transcription
Up-regulation
Viruses
α-Interferon
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is scarcely addressed. The aim of this work was to evaluate the differential expression of genes involved in immune and inflammatory response. Groups of 20–25 embryos on Day 6 (morula stage) were exposed (infected) or not (control) to an NCP-BVDV strain in SOF medium. After 24 h, embryos that reached expanded blastocyst stage were washed. Total RNA of each embryo group was extracted to determine the transcription levels of 9 specific transcripts related with antiviral and inflammatory response by SYBR Green real time quantitative (RT-qPCR). Culture media and an aliquot of the last embryos wash on Day 7 were analyzed by titration and virus isolation, respectively. A conventional PCR confirmed BVDV presence in IVP embryos. A significantly higher expression of interferon-α was observed in blastocysts exposed to NCP-BVDV compared to the controls (p 
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-020-05958-7