Follicular Fluid Metabolite Changes in Dairy Cows with Inactive Ovary Identified Using Untargeted Metabolomics

• Published in: BioMed research international 2020, Vol.2020 (2020), p.1-10
• Main Authors: Wu, Ling, Xu, Chuang, Zhang, HongYou, Zhang, Feng, Bai, YunLong, Xia, Cheng
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Animal lactation; Animals; Arachidonic acid; Biosynthesis; Carbohydrates; Cattle; Chromatography; Chromatography, Liquid; Dairy cattle; Dairy farms; Dairying; Data Analysis; Discriminant Analysis; Diseases; Efficiency; Energy; Estrus; Female; Follicular fluid; Follicular Fluid - metabolism; Genetic aspects; Glycerol; Growth; Insulin-like growth factors; Isoleucine; Least-Squares Analysis; Leucine; Lipid metabolism; Lipids; Mass Spectrometry; Metabolic Networks and Pathways; Metabolic pathways; Metabolism; Metabolites; Metabolome; Metabolomics; Methods; Milk; Molecular mechanics; Molecular modelling; Multivariate statistical analysis; Nucleotides; Ovaries; Ovary - metabolism; Phenylalanine; Phospholipids; Postpartum; Principal Component Analysis; Principal components analysis; Scientific imaging; Statistical analysis; Statistical methods; Sugar; Systems stability; Technology assessment; Valine
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2020/9837543

The metabolism of dairy cows with inactive ovaries differs from that of healthy dairy cows. However, the molecular mechanisms underpinning these physiological and metabolic changes remain unclear. The purpose of this study was to investigate follicular fluid metabolite changes in dairy cows with inactive ovaries. Untargeted metabolomics technology and multivariate statistical analysis were used to screen differential metabolites in follicular fluid samples between inactive ovaries and estrus cows at 45-60 d postpartum. Fourteen differential metabolites were identified, consisting of amino acids, lipids, sugars, and nucleotides. When compared with healthy animal samples, eight follicular fluid metabolites were significantly increased, and six metabolites were significantly decreased in dairy cows with inactive ovaries. Metabolic pathway analyses indicated that differential metabolites were primarily involved in glycerol phospholipid metabolism, arachidonic acid metabolism, valine, leucine and isoleucine biosynthesis, and phenylalanine metabolism. These metabolites and their enrichment pathways indicate that the enhancement of lipid metabolism and the weakening of carbohydrate production of amino acids in dairy cows with impaired follicular development. Overall, these data provide a better understanding of the changes that could affect follicular development during the postpartum period and lay the ground for further investigations.