Quantitative trait loci (QTL) associated with resistance of rainbow trout Oncorhynchus mykiss against the parasitic ciliate Ichthyophthirius multifiliis

• Published in: Journal of fish diseases 2020-12, Vol.43 (12), p.1591-1602
• Main Authors: Jaafar, R, Ødegård, J, Mathiessen, H, Karami, A M, Marana, M H, Gersdorff Jørgensen, L, Zuo, S, Nielsen, T, Kania, P W, Buchmann, K
• Format: Article
• Language: English
• Subjects: Animals; Aquaculture; Aquaculture enterprises; Biomarkers; Cathelicidins; Chromosomes; Ciliophora Infections - immunology; Ciliophora Infections - veterinary; Cultured organisms; Deoxyribonucleic acid; disease; DNA; DNA sequences; Economic impact; Economics; Fish; Fish culture; Fish diseases; Fish Diseases - parasitology; Freshwater; Freshwater fish; Freshwater fishes; Gene expression; Gene Expression Regulation - immunology; Gene mapping; Genes; Genome; Genomes; Host specificity; Husbandry diseases; Hymenostomatida; Ichthyophthirius multifiliis; Immunity, Innate - genetics; Impact analysis; Infections; Inland water environment; Morbidity; Nucleotides; Oncorhynchus mykiss; Oncorhynchus mykiss - genetics; Oncorhynchus mykiss - immunology; Oncorhynchus mykiss - parasitology; Original; Parasites; Polymorphism, Single Nucleotide; Protozoa; Quantitative Trait Loci; Receptors; resistance; Salmon; selective breeding; Selective Breeding - genetics; Single-nucleotide polymorphism; Specificity; Survival; susceptibility; T cell receptors; Trout; White spot disease
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.13264

The parasitic ciliate Ichthyophthirius multifiliis has a low host specificity eliciting white spot disease (WSD) in a wide range of freshwater fishes worldwide. The parasite multiplies rapidly whereby the infection may reach problematic levels in a host population within a few days. The parasite targets both wild and cultured fish but the huge economic impact of the protozoan is associated with mortality, morbidity and treatment in aquacultural enterprises. We have investigated the potential for genetic selection of WSD‐resistant strains of rainbow trout. Applying the DNA typing system Affymetrix® and characterizing the genome of the individual fish by use of 57,501 single nucleotide polymorphisms (SNP) and their location on the rainbow trout chromosomes, we have genetically characterized rainbow trout with different levels of natural resistance towards WSD. Quantitative trait loci (QTL) used for the selection of breeders with specific markers for resistance are reported. We found a significant association between resistance towards I. multifiliis infection and SNP markers located on the two specific rainbow trout chromosomes Omy 16 and Omy 17. Comparing the expression of immune‐related genes in fish—with and without clinical signs—we recorded no significant difference. However, trout surviving the infection showed high expression levels of genes encoding IgT, T‐cell receptor TCRβ, C3, cathelicidins 1 and 2 and SAA, suggesting these genes to be associated with protection.