Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut ( Castanea sativa ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied -woody plant interactions and identify molecular me...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2020-11, Vol.21 (22), p.8525
Main Authors: Saiz-Fernández, Iñigo, Milenković, Ivan, Berka, Miroslav, Černý, Martin, Tomšovský, Michal, Brzobohatý, Břetislav, Kerchev, Pavel
Format: Article
Language:English
Subjects:
Binding Sites
Castanea sativa
Chestnut
Computational Biology
Cyclopentanes - metabolism
Defense mechanisms
Enzymes
Fagaceae - metabolism
Fagaceae - microbiology
Flowers & plants
Hardwoods
Homeostasis
Host range
Infections
Inoculation
Jasmonic acid
Kinases
Metabolism
Metabolomics
Molecular modelling
Oxylipins - metabolism
Pathogenicity
Pathogens
Peptides
Phytophthora - pathogenicity
Phytophthora cinnamomi
Plant Diseases - microbiology
Plant Growth Regulators - metabolism
Plant Roots
Processing industry
Proteins
Proteomes
Proteomics
Salicylic acid
Salicylic Acid - metabolism
Signal Transduction
Stems
Trees
Wood
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied -woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between and sweet chestnut ( ), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of attack on stem tissues immediately bordering the infection site and away from it. led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in pathogenicity.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21228525