HGG-37. PAEDIATRIC GLIOBLASTOMA CELLS SHOW CRITICAL DEPENDENCIES ON EPIGENOMIC AND EPITRANSCRIPTOMIC CONTROL OF GENE EXPRESSION BY H3.3G34R/V MUTATIONS

Abstract H3.3G34R/V mutations are restricted to glioblastomas of the cerebral hemispheres, and occur predominantly in adolescents and young adults. We had previously shown these mutations to result in a global re-organisation of the activating mark H3K36me3 to drive transcription of key developmenta...

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Published in:Neuro-oncology (Charlottesville, Va.) Va.), 2020-12, Vol.22 (Supplement_3), p.iii350-iii350
Main Authors: Bjerke, Lynn, Mackay, Alan, Rogers, Rebecca, Grabovska, Yura, Molinari, Valeria, Temelso, Sara, Cole, Kristina, Waanders, Angela, Carcaboso, Angel Montero, Vinci, Maria, Jones, Chris
Format: Article
Language:English
Subjects:
High Grade Glioma
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Summary:Abstract H3.3G34R/V mutations are restricted to glioblastomas of the cerebral hemispheres, and occur predominantly in adolescents and young adults. We had previously shown these mutations to result in a global re-organisation of the activating mark H3K36me3 to drive transcription of key developmental transcription factors and oncogenes such as MYCN, however the precise mechanism was unclear. Using multiple H3G34R/V samples and ChIP-seq with antibodies specific to both wild-type and mutant histone H3.3, we show a high degree of incorporation of mutant histone into nucleosomes, with only a minority (
ISSN:1522-8517
1523-5866
DOI:10.1093/neuonc/noaa222.318