Advanced co-culture 3D breast cancer model for investigation of fibrosis induced by external stimuli: optimization study

Radiation-induced fibrosis (RIF) is the main late radiation toxicity in breast cancer patients. Most of the current 3D in vitro breast cancer models are composed by cancer cells only and are unable to reproduce the complex cellular homeostasis within the tumor microenvironment to study RIF mechanism...

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Bibliographic Details
Published in:Scientific reports 2020-12, Vol.10 (1), p.21273-21273, Article 21273
Main Authors: Yakavets, Ilya, Francois, Aurelie, Benoit, Alice, Merlin, Jean-Louis, Bezdetnaya, Lina, Vogin, Guillaume
Format: Article
Language:English
Subjects:
631/1647/767/70
631/67/1059/485
631/67/1347
Breast cancer
Breast Neoplasms
Cancer
Cell culture
Coculture Techniques
Culture media
External stimuli
Extracellular matrix
Fibroblasts
Fibrosis
Homeostasis
Humanities and Social Sciences
Humans
Immunohistochemistry
Irradiation
Life Sciences
MCF-7 Cells
Models, Biological
multidisciplinary
Science
Science (multidisciplinary)
Spheroids
Spheroids, Cellular
Stroma
Toxicity
Tumor Cells, Cultured
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Summary:Radiation-induced fibrosis (RIF) is the main late radiation toxicity in breast cancer patients. Most of the current 3D in vitro breast cancer models are composed by cancer cells only and are unable to reproduce the complex cellular homeostasis within the tumor microenvironment to study RIF mechanisms. In order to account complex cellular interactions within the tumor microenvironment, an advanced 3D spheroid model, consisting of the luminal breast cancer MCF-7 cells and MRC-5 fibroblasts, was developed. The spheroids were generated using the liquid overlay technique in culture media into 96-well plates previously coated with 1% agarose (m/v, in water). In total, 21 experimental setups were tested during the optimization of the model. The generated spheroids were characterized using fluorescence imaging, immunohistology and immunohistochemistry. The expression of ECM components was confirmed in co-culture spheroids. Using α-SMA staining, we confirmed the differentiation of healthy fibroblasts into myofibroblasts upon the co-culturing with cancer cells. The induction of fibrosis was studied in spheroids treated 24 h with 10 ng/mL TGF-β and/or 2 Gy irradiation. Overall, the developed advanced 3D stroma-rich in vitro model of breast cancer provides a possibility to study fibrosis mechanisms taking into account 3D arrangement of the complex tumor microenvironment.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-78087-7