Establishment and characterization of a canine sebaceous epithelial cell line derived from an eyelid mass

• Published in: Journal of Veterinary Medical Science 2020, Vol.82(11), pp.1577-1584
• Main Authors: MATSUDA, Akira, MITSUI, Ikki, SHIMIZU, Yuki, KANDA, Teppei, OHNISHI, Akihiro, MIYABE, Masahiro, ITOH, Yoshiki
• Format: Article
• Language: English
• Subjects: Cell culture; cell line; Cell proliferation; Chemokines; Cytoplasm; dog; Epithelial cells; Eyelid; IL-1β; Inflammation; Interleukin 8; Internal Medicine; Linoleic acid; Lipogenesis; Lipopolysaccharides; meibomian gland; sebaceous epithelial cell; Sebaceous gland; Skin diseases; Tissue culture; Transforming growth factor-b; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.20-0179

Little is known about the pathological roles of sebaceous glands in canine skin diseases, as most examinations have been conducted with cultured human sebaceous epithelial cell lines. To our knowledge, there is no available canine sebaceous epithelial cell line. The purpose of this study was to establish a canine sebaceous epithelial cell line and characterize it. An eyelid mass in a dog was surgically resected for treatment, and it was histologically diagnosed as sebaceous epithelioma. Collected tissue was conducted for culture, and the growing epithelial-like cells were passaged. The cells showed continuous proliferation for over 6 months. After 40 passages, the cells were named CMG-1. Lipid droplets in the cytoplasm of CMG-1 cells were confirmed by Oil Red O staining. As reported in studies with human sebaceous epithelial cell lines, lipogenesis in CMG-1 cells was promoted by linoleic acid, whereas transforming growth factor-β (TGF-β) suppressed it. Additionally, real-time PCR revealed that the expression levels of chemokines and cytokines, including CC chemokine ligand (CCL)-2, CCL-20, CXCL-10, Tumor necrosis factor-α (TNF-α), Interleukin (IL)-1α, IL-1β, and IL-8, were significantly increased in CMG-1 cells following treatment with lipopolysaccharide. In conclusion, we successfully established a new canine sebaceous epithelial cell line. Our data indicated that lipogenesis and inflammatory responses were quantitatively evaluable in this cell line. CMG-1 cells could be useful for the pathological analysis of sebaceous gland diseases in dogs.