Expression of miRNAs in plasma exosomes derived from patients with atrial fibrillation

• Published in: Clinical cardiology (Mahwah, N.J.) N.J.), 2020-12, Vol.43 (12), p.1450-1459
• Main Authors: Wei, Zhang, Bing, Zhang, Shaohuan, Qian, Yanran, Wang, Shuo, Sun, Bi, Tang, Feiyu, Zhu, Heng, Zhang, Qin, Gao, Pinfang, Kang
• Format: Article
• Language: English
• Subjects: atrial fibrillation (AF); Cardiac arrhythmia; Cardiovascular disease; Clinical Investigations; Electrocardiography; exosomes; Gene expression; Heart failure; high‐throughput sequencing; Hypertension; Medical research; MicroRNAs; miRNA; Plasma; Proteins; Statistical analysis
• ISSN: 0160-9289; 1932-8737
• DOI: 10.1002/clc.23461

Background Studies have revealed the association between exosomes and cardiovascular diseases. However, the typical changes of plasma miRNAs in patients with atrial fibrillation (AF) are still controversial, the use of exosomal miRNAs to diagnose and predict the prognosis of AF has not been described. Hypothesis We hypothesized that there were differences in the exosomal miRNAs between AF and normal sinus rhythm (SR) patients, which might be used as the novel biomarkers to reflect the progression of AF. Methods miRNAs were isolated from the plasma of patients, and the target genes of differential miRNAs via enrichment analysis to discover potential pathogenesis related to AF. Combined with high‐throughput sequencing results, real‐time PCR was used to verify the relative expression of target miRNAs in patients. Results This study confirmed that the expression of plasma‐derived exosomal miRNAs between patients with AF and SR were different. Target gene enrichment analysis suggested that the target genes of 20 miRNAs, which were significantly upregulated were mainly enriched in biological processes such as gene expression process, inflammation response, enzyme modification, etc. Meanwhile, mitogen‐activated protein kinase (MAPK), mammalian target of rapamycin (mTOR), and other pathways were highly enriched. The expressions of miR‐92b‐3p, miR‐1306‐5p, and miR‐let‐7b‐3p had differences between patients with AF and SR. Conclusion These miRNAs and target genes were involved in the process of AF through affecting biological processes such as energy metabolism, lipid metabolism, inflammation, and enzyme activity. It suggested that the exosomal miRNAs might be used as the novel biomarkers to reflect the progression of AF.