Differential Distribution of RBPMS in Pig, Rat, and Human Retina after Damage

RNA binding protein with multiple splicing (RBPMS) is expressed exclusively in retinal ganglion cells (RGCs) in the retina and can label all RGCs in normal retinas of mice, rats, guinea pigs, rabbits, cats, and monkeys, but its function in these cells is not known. As a result of the limited knowled...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-12, Vol.21 (23), p.9330
Main Authors: Pereiro, Xandra, Ruzafa, Noelia, Urcola, J Haritz, Sharma, Sansar C, Vecino, Elena
Format: Article
Language:English
Subjects:
Animals
Axons
Axons - metabolism
Axons - pathology
Cell culture
Cell Hypoxia
Cells, Cultured
Degeneration
Dendrites
Explants
Gene expression
Guinea pigs
Humans
Hypoxia
Labeling
Localization
Mammals
Monkeys
Neonates
Neurodegeneration
Neurogenesis
Optic nerve
Protein Transport
Proteins
Rabbits
Rats
Retina
Retinal ganglion cells
Retinal Ganglion Cells - cytology
Retinal Ganglion Cells - metabolism
Retinal Ganglion Cells - pathology
RNA-binding protein
RNA-Binding Proteins - metabolism
Splicing
Swine
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Summary:RNA binding protein with multiple splicing (RBPMS) is expressed exclusively in retinal ganglion cells (RGCs) in the retina and can label all RGCs in normal retinas of mice, rats, guinea pigs, rabbits, cats, and monkeys, but its function in these cells is not known. As a result of the limited knowledge regarding RBPMS, we analyzed the expression of RBPMS in the retina of different mammalian species (humans, pigs, and rats), in various stages of development (neonatal and adult) and with different levels of injury (control, hypoxia, and organotypic culture or explants). In control conditions, RBPMS was localized in the RGCs somas in the ganglion cell layer, whereas in hypoxic conditions, it was localized in the RGCs dendrites in the inner plexiform layer. Such differential distributions of RBPMS occurred in all analyzed species, and in adult and neonatal retinas. Furthermore, we demonstrate RBPMS localization in the degenerating RGCs axons in the nerve fiber layer of retinal explants. This is the first evidence regarding the possible transport of RBPMS in response to physiological damage in a mammalian retina. Therefore, RBPMS should be further investigated in relation to its role in axonal and dendritic degeneration.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21239330