Activation of LKB1 rescues 3T3-L1 adipocytes from senescence induced by Sirt1 knock-down: a pivotal role of LKB1 in cellular aging

Previous reports have shown that excess calorie intake promotes p53 dependent senescence in mouse adipose tissues. The objective of the current study was to address the mechanism underlying this observation, i.e. adipocyte aging. Using cultured 3T3-L1 cells, we investigated the involvement of energy...

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Published in:Aging (Albany, NY.) NY.), 2020-10, Vol.12 (19), p.18942-18956
Main Authors: Lan, Fan, Lin, Yan, Gao, Zhenfeng, Cacicedo, Jose M, Weikel, Karen, Ido, Yasuo
Format: Article
Language:English
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Research Paper
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Summary:Previous reports have shown that excess calorie intake promotes p53 dependent senescence in mouse adipose tissues. The objective of the current study was to address the mechanism underlying this observation, i.e. adipocyte aging. Using cultured 3T3-L1 cells, we investigated the involvement of energy regulators Sirt1, AMPK, and LKB1 in senescence. Fifteen days post differentiation, Sirt1 knock-down increased senescence-associated beta-galactosidase (SA-β-Gal) staining by 20-40% (p
ISSN:1945-4589
1945-4589
DOI:10.18632/aging.104052