A New Method for the Detection of Colorectal Cancer and the Precancerous Lesions: Occult Blood Testing Combination with Promoter Methylation in the Fecal Sample

Noninvasive stool-based DNA methylation testing emerges as a new approach for detecting colorectal cancer (CRC). However, its feasibility for early detection of CRC and precancerous lesions in the Chinese population remains inconclusive. In this study, we establish a possibilities screening method (...

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Bibliographic Details
Published in:Journal of Cancer 2021, Vol.12 (2), p.335-342
Main Authors: Wang, Dan-Yang, He, Kang-Xin, Huang, Ying, Lou, Qin-Qin, He, Ti, Xu, Xiao
Format: Article
Language:English
Subjects:
Blood tests
Colonoscopy
Colorectal cancer
DNA methylation
Feces
Genes
Genetic testing
Research Paper
Thermal cycling
Tumors
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Summary:Noninvasive stool-based DNA methylation testing emerges as a new approach for detecting colorectal cancer (CRC). However, its feasibility for early detection of CRC and precancerous lesions in the Chinese population remains inconclusive. In this study, we establish a possibilities screening method (sDNA-FOBT) for detecting CRC and precancerous lesions (hyperplastic polyps [HP] and adenomas [AD]) and evaluate its detection performance in the Chinese population. This method combined a molecular assay of DNA methylation markers (BMP3, NDRG4, and SDC2) with the human hemoglobin test (FOBT) in stool samples. The sensitivity of sDNA-FOBT was 85.42% for CRC, 85.71% for AD, and 28.21% for HP, respectively, at the specificity of 92%. The diagnostic efficacy of sDNA-FOBT for detecting CRC and precancerous lesions was significantly higher than FOBT alone (sensitivity: 61.70% vs. 51.06%,
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.50525