Detection of miR-1246, miR-23a and miR-451 in sera of colorectal carcinoma patients: a case-control study in Cairo University hospital

• Published in: African health sciences 2020-09, Vol.20 (3), p.1283-1291
• Main Authors: Salah, Mona, Shaheen, Iman, El-Shanawany, Pakinam, Saad, Nagwa Eid, Saad, Rasha, El Guibaly, Mahmoud, Momen, Nouran
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biomarkers, Tumor - blood; Case-Control Studies; Colonic Neoplasms - blood; Colonic Neoplasms - diagnosis; Colonic Neoplasms - genetics; Colonic Neoplasms - pathology; Colorectal Neoplasms - blood; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Egypt; Female; Humans; Male; MicroRNAs - blood; Middle Aged; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity
• ISSN: 1680-6905; 1729-0503; 1680-6905
• DOI: 10.4314/ahs.v20i3.33

Colorectal cancer (CRC) has high morbidity and mortality rates. Invasive techniques and other laboratory tests with variable sensitivity and specificity are currently used in diagnosis. Micro ribonucleic acids (miRNAs) have bio vital roles in cell proliferation and apoptosis. Dys-regulation of miRNAs is linked to tumour genesis. The objective of this study was to evaluate the specificity and sensitivity of serum non-invasive biomarkers (micro-RNAs), miR-1246, miR-23a, and miR-451in CRC patients. Peripheral expression of three miRNAs (miR-1246, miR-23a and miR-451) was investigated in sera of 37 CRC Egyptian patients and 30 healthy controls, using quantitative real-time polymerase chain reaction trying to reach the optimal non-invasive combination of miRNAs. Serum miR-1246 was up-regulated in sera of CRC patients compared to normal controls (fold change = 3.55; P