Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells

A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorop...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2020-11, Vol.59 (48), p.21431-21435
Main Authors: Robson, Jonathan A., Kubánková, Markéta, Bond, Tamzin, Hendley, Rian A., White, Andrew J. P., Kuimova, Marina K., Wilton‐Ely, James D. E. T.
Format: Article
Language:English
Subjects:
Biocompatibility
Boron Compounds - chemistry
Carbon monoxide
Carbon Monoxide - analysis
Communication
Communications
Coordination Complexes - chemistry
endogenous CO
Fluorescence
Fluorescent Dyes - chemistry
Humans
Hypoxia
Mammalian cells
MCF-7 Cells
Molecular Structure
Optical Imaging
Organelles
Probes
Ruthenium
Ruthenium compounds
sensing
Viscosity
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Summary:A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells. Carbon monoxide (CO) is produced in mammals and regulates many critical cellular functions, including the response to disease. A ruthenium(II) vinyl probe is presented that allows rapid, selective and sensitive detection of endogenous CO in live mammalian cells under normoxic and hypoxic conditions. By exploiting fluorescence lifetime imaging microscopy, the probe can also measure microscopic viscosity in live cells at the same time.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202008224