Reductive Charge Transfer through an RNA Aptamer

The transfer of charges through double helical DNA is a very well investigated bioelectric phenomenon. RNA, on the contrary, has been less studied in this regard. The few available data report on charge transfer through RNA duplex structures mainly composed of homonucleotide sequences. In the light...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2020-12, Vol.59 (51), p.22999-23004
Main Authors: Frommer, Jennifer, Müller, Sabine
Format: Article
Language:English
Subjects:
Aptamers
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Base Pairing
Bioelectricity
Charge transfer
Communication
Communications
Deoxyribonucleic acid
DNA
Double-stranded
Electron Transport
fluorescence quenching
Gene sequencing
Nucleic Acid Conformation
optical spectroscopy
Oxidation-Reduction
pyrene-modified RNA
Ribonucleic acid
RNA
RNA aptamers
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Summary:The transfer of charges through double helical DNA is a very well investigated bioelectric phenomenon. RNA, on the contrary, has been less studied in this regard. The few available data report on charge transfer through RNA duplex structures mainly composed of homonucleotide sequences. In the light of the RNA world scenarios, it is an interesting question, if charge transfer can be coupled with RNA function. Functional RNAs however, contain versatile structural motifs. Therefore, electron transport also through non‐Watson–Crick base‐paired regions might be required. We here demonstrate distance‐dependent reductive charge transfer through RNA duplexes and through the non‐Watson–Crick base‐paired region of an RNA aptamer. RNA charge transfer: Reductive charge transfer through regular RNA duplexes and through a RNA aptamer is demonstrated. Charge transfer is induced by photochemical activation of 5‐dimethylaminopyreneuridine, incorporated in RNA as an electron donor. Charge transfer proceeds in a distance‐dependent manner and was monitored by fluorescence quenching and dehalogenation of 5‐bromodeoxyuridine, used as an electron trap.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202009430