Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells

Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointest...

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Published in:International journal of molecular sciences 2020-12, Vol.21 (24), p.9347
Main Authors: Mori, Shiori, Kishi, Shingo, Honoki, Kanya, Fujiwara-Tani, Rina, Moriguchi, Takuma, Sasaki, Takamitsu, Fujii, Kiyomu, Tsukamoto, Shinji, Fujii, Hiromasa, Kido, Akira, Tanaka, Yasuhito, Luo, Yi, Kuniyasu, Hiroki
Format: Article
Language:English
Subjects:
Acetylcysteine
Acetylcysteine - pharmacology
Animals
Antineoplastic Agents - pharmacology
Antioxidants
Antitumor activity
Apoptosis
Ataxia
Autophagy
Blueberries
Cancer
Cell cycle
Cell growth
Cell Proliferation
Cytotoxicity
DNA methylation
Gastrointestinal cancer
Gastrointestinal Neoplasms - metabolism
Gene expression
HT29 Cells
Humans
Ischemia
Kinases
Lipid Peroxidation
Lipids
Membrane potential
Metastasis
Mice
MicroRNAs
Mitochondria
Neoplastic Stem Cells - drug effects
Oxidative stress
Permeability
Peroxide
Proteins
Signal transduction
Stem cells
Stilbenes - pharmacology
Tumor cell lines
Vitamin E
Vitamin E - pharmacology
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Summary:Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell lines, namely CT26, HT29, and MKN74. PTE showed a dose-dependent inhibition of cell proliferation, sphere-forming ability, and stem cell marker expression in all three cell lines. Furthermore, the cells treated with PTE showed an increase in mitochondrial membrane potential and an increase in mitochondrial oxidative stress and lipid peroxide. Upon concurrent treatment with vitamin E, N-acetyl-L-cysteine, and PTE, the PTE-induced mitochondrial oxidative stress and growth inhibition were suppressed. These findings indicate that PTE induces oxidative stress in cancer cells, suppresses stemness, and inhibits proliferation. These antitumor effects of PTE are considered to be useful in cancer treatment.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21249347