Chronic Rhinosinusitis: MALDI-TOF Mass Spectrometry Microbiological Diagnosis and Electron Microscopy Analysis; Experience of the 2nd Otorhinolaryngology Clinic of Cluj-Napoca, Romania

(1) Background: Chronic rhinosinusitis (CRS) represents a wide range of infectious-inflammatory processes affecting, simultaneously, the nose and paranasal sinuses mucosa. The paper presents outcomes of the investigation of CRS microbiological characteristics in a group of 32 patients. (2) Methods:...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical medicine 2020-12, Vol.9 (12), p.3973
Main Authors: Jeican, Ionuț Isaia, Barbu Tudoran, Lucian, Florea, Adrian, Flonta, Mirela, Trombitas, Veronica, Apostol, Anda, Dumitru, Mihai, Aluaș, Maria, Junie, Lia Monica, Albu, Silviu
Format: Article
Language:English
Subjects:
Biofilms
Chronic illnesses
Clinical medicine
Disease
Endoscopy
Inflammation
Ions
Laboratories
Mass spectrometry
Microorganisms
Otolaryngology
Patients
Polymerization
Polyps
Rhinitis
Scanning electron microscopy
Scientific imaging
Sinuses
Sinusitis
Surgery
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:(1) Background: Chronic rhinosinusitis (CRS) represents a wide range of infectious-inflammatory processes affecting, simultaneously, the nose and paranasal sinuses mucosa. The paper presents outcomes of the investigation of CRS microbiological characteristics in a group of 32 patients. (2) Methods: The purulent samples were collected during functional endoscopic sinus surgery. Agar plates were incubated and examined. All types of colonies were identified using Matrix-Assisted Laser Desorption - Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS). For scanning electron microscopy, samples were fixed and sputter-coated with 10 nm gold and analyzed using a scanning electron microscope. For transmission electron microscopy, samples were fixed, postfixed, and dehydrated. After polymerization, ultrathin sections were collected on carbon coated copper grids and analyzed with Jeol JEM1010 TEM. (3) Results: Positive microbiological diagnosis was obtained in 62.5% of cases. The most frequent species found are and subsp. . Corynebacterium aurimucosum and Eggerthia catenaformis were unreported species in CRS until the present. Biofilm was evidenced in 43.7% of sinus mucosa samples. Ciliary disorientation, atrophy, and no ciliated cells were also identified. (4) Conclusion: The microbial factor-pathogen or opportunistic-is one of the most important pathological links in chronic rhinosinusitis. MALDI-TOF MS allows easily and quickly identification of germs.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm9123973