Porphyromonas gingivalis Mfa1 Induces Chemokine and Cell Adhesion Molecules in Mouse Gingival Fibroblasts via Toll-Like Receptors

Mfa1 fimbriae are thought to act as adhesion factors and to direct periodontal tissue destruction but their immunomodulatory actions are poorly understood. Here, we investigated the effect of Mfa1 stimulation on the immune and metabolic mechanisms of gingival fibroblasts from periodontal connective...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical medicine 2020-12, Vol.9 (12), p.4004
Main Authors: Takayanagi, Yuhei, Kikuchi, Takeshi, Hasegawa, Yoshiaki, Naiki, Yoshikazu, Goto, Hisashi, Okada, Kousuke, Okabe, Iichiro, Kamiya, Yosuke, Suzuki, Yuki, Sawada, Noritaka, Okabe, Teppei, Kondo, Shun, Ohno, Tasuku, Hayashi, Jun-Ichiro, Mitani, Akio
Format: Article
Language:English
Subjects:
Bacteria
Biofilms
Cell adhesion & migration
Clinical medicine
Fibroblasts
Flow cytometry
Gene expression
Laboratories
Metabolism
Pathogens
Proteins
Software
Variance analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mfa1 fimbriae are thought to act as adhesion factors and to direct periodontal tissue destruction but their immunomodulatory actions are poorly understood. Here, we investigated the effect of Mfa1 stimulation on the immune and metabolic mechanisms of gingival fibroblasts from periodontal connective tissue. We also determined the role of Toll-like receptor (TLR) 2 and TLR4 in Mfa1 recognition. Mfa1 increased the expression of genes encoding chemokine (C-X-C motif) ligand (CXCL) 1, CXCL3, intercellular adhesion molecule (ICAM) 1 and Selectin endothelium (E) in gingival fibroblasts, but did not have a significant effect on genes that regulate metabolism. Mfa1-stimulated up-regulation of genes was significantly suppressed in siRNA-transfected cells compared with that in control siRNA-transfected cells, which indicates that recognition by TLR4 is essential for immunomodulation by Mfa1. Additionally, suppression of expression partially attenuated the stimulatory effect of Mfa1. Overall, these results help explain the involvement of Mfa1 fimbriae in the progression of periodontal disease.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm9124004