Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato

• Published in: International journal of molecular sciences 2020-12, Vol.21 (24), p.9380
• Main Authors: Nikoloudakis, Nikolaos, Pappi, Polyxeni, Markakis, Emmanouil A, Charova, Spyridoula N, Fanourakis, Dimitrios, Paschalidis, Konstantinos, Delis, Costas, Tzortzakakis, Emmanuel A, Tsaniklidis, Georgios
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Antiinfectives and antibacterials; Antimicrobial peptides; Cold-Shock Response; Defensins; Defensins - genetics; Defensins - metabolism; Eukaryotes; Gene Expression Regulation, Plant; Gene regulation; Genes; Genomes; Genomics; Host-Pathogen Interactions; Infections; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; Lycopersicon esculentum - microbiology; Lycopersicon esculentum - parasitology; Meloidogyne javanica; Microorganisms; Nucleotides; Pathogens; Pattern recognition; Peptides; Plant Proteins - genetics; Plant Proteins - metabolism; Plant roots; Plant virus diseases; Potatoes; Potyviridae; Proteins; Solanum lycopersicum; Transcription factors; Transcriptional Activation; Tylenchoidea - pathogenicity; Verticillium - pathogenicity; Verticillium dahliae; Viral infections
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21249380

Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are widespread across eukaryotes, still, extensive nucleotide and amino acid dissimilarities hamper the elucidation of their response to stimuli and mode of function. In the current study, we screened the genome for the identification of defensin genes, predicted the relating protein structures, and further studied their transcriptional responses to biotic ( , , , and infections) and abiotic (cold stress) stimuli. Tomato defensin sequences were classified into two groups (C8 and C12). Our data indicate that the transcription of defensin coding genes primarily depends on the specific pathogen recognition patterns of and . The immunodetection of plant defensin 1 protein was achieved only in the roots of plants inoculated with In contrast, the almost null effects of viral infections and cold stress, and the failure to substantially induce the gene transcription suggest that these factors are probably not primarily targeted by the tomato defensin network.