Developing a novel artificial rice germplasm for dinitroaniline herbicide resistance by base editing of OsTubA2

• Published in: Plant biotechnology journal 2021-01, Vol.19 (1), p.5-7
• Main Authors: Liu, Lang, Kuang, Yongjie, Yan, Fang, Li, Shaofang, Ren, Bin, Gosavi, Gokul, Spetz, Carl, Li, Xiangju, Wang, Xifeng, Zhou, Xueping, Zhou, Huanbin
• Format: Article
• Language: English
• Subjects: Amino acids; base editing; Brief Communication; Brief Communications; Cell division; Crop production; Crops; Deoxyribonucleic acid; dinitroaniline; DNA; Domestication; Editing; Gene Editing; Genes; Genetic engineering; Genomes; Germplasm; Herbicide resistance; Herbicide Resistance - genetics; Herbicides; Herbicides - pharmacology; Inhibitors; Mutation; Oryza - genetics; OsTubA2; Plant breeding; Plants, Genetically Modified - genetics; Point mutation; Rice; Seeds; Sustainable agriculture; Sustainable production; Tubulin; Weed control; Weeds
• ISSN: 1467-7644; 1467-7652
• DOI: 10.1111/pbi.13430

[...]the use of chemical herbicides (i.e. ALS inhibitors, EPSPS inhibitors, ACCase inhibitors) has also drastically increased, resulting in the occurrence of weed species resistant to these herbicides. [...]developing novel herbicide‐resistant crops to diversify weed management is of great value in slowing the evolution of weed resistance to herbicides and to maintain sustainable crop production in the future. [...]tubulin genes are promising target genes to develop herbicide‐resistant germplasms for future crop breeding. Proteins belonging to the α‐tubulin and β‐tubulin family are highly conserved, showing up to 88% amino acid similarity (Rao et al., 2016). [...]we hypothesized that introduction of this point mutation into the rice genome might transform common rice into a herbicide‐tolerant variety. The data indicate that M268 in OsTubA2 has not been targeted by natural or human selection during rice domestication (Figure 1k). [...]M268T plants are a novel artificial germplasm with a great potential for future rice improvement.