Single-cell Sequencing in the Field of Stem Cells

Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional sequencing analysis methods often cover up this difference. Single-cell sequencing technology refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It can...

Full description

Saved in:
Bibliographic Details
Published in:Current genomics 2020-12, Vol.21 (8), p.576-584
Main Authors: Chen, Tian, Li, Jiawei, Jia, Yichen, Wang, Jiyan, Sang, Ruirui, Zhang, Yi, Rong, Ruiming
Format: Article
Language:English
Subjects:
Gene sequencing
Genomes
Heterogeneity
Next-generation sequencing
Nucleic acids
Pluripotency
Sequence analysis
Stem cells
Technology assessment
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional sequencing analysis methods often cover up this difference. Single-cell sequencing technology refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It can effectively analyze cell heterogeneity and identify a small number of cell populations. With the continuous progress of cell sorting, nucleic acid extraction and other technologies, single-cell sequencing technology has also made great progress. Encouraging new discoveries have been made in stem cell research, including pluripotent stem cells, tissue-specific stem cells and cancer stem cells. In this review, we discuss the latest progress and future prospects of single-cell sequencing technology in the field of stem cells.
ISSN:1389-2029
1875-5488
DOI:10.2174/1389202921999200624154445