Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes

BACKGROUND This study was carried out to analyze TOP2A expression in lung adenocarcinoma (LUAD) and to assess its value in clinical diagnosis and prognosis. MATERIAL AND METHODS The Cancer Genome Atlas (TCGA) database was used to study the relationship of TOP2A expression with the progression and pr...

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Published in:Medical science monitor 2020-12, Vol.26, p.e929120-e929120
Main Authors: Du, Xiaomei, Xue, Zhiwen, Lv, Jianning, Wang, Heidou
Format: Article
Language:English
Subjects:
A549 Cells
Adenocarcinoma of Lung - genetics
Adenocarcinoma of Lung - pathology
Database Analysis
DNA Topoisomerases, Type II - genetics
DNA Topoisomerases, Type II - metabolism
Down-Regulation - genetics
Gene Expression Regulation, Neoplastic
Genetic Association Studies
Humans
Lung Neoplasms - genetics
Lung Neoplasms - pathology
MAP Kinase Signaling System - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Treatment Outcome
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Xue, Zhiwen
Lv, Jianning
Wang, Heidou
description BACKGROUND This study was carried out to analyze TOP2A expression in lung adenocarcinoma (LUAD) and to assess its value in clinical diagnosis and prognosis. MATERIAL AND METHODS The Cancer Genome Atlas (TCGA) database was used to study the relationship of TOP2A expression with the progression and prognosis of LUAD. For a further elucidation of the value of TOP2A in LUAD, the effect of TOP2A knockout on cell viability and related protein expression of LUAD cell line A549 in vitro was investigated by using RNA interference, MTT, flow cytometry, RT-PCR, and western blot analysis. RESULTS According to the results of database analysis, TOP2A expression in LUAD was higher than that in normal lung tissues. There was a strong correlation of TOP2A expression with clinicopathological and epidemiological parameters of LUAD. The survival rate of LUAD patients with high TOP2A expression was lower than that of patients with low expression (P
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MATERIAL AND METHODS The Cancer Genome Atlas (TCGA) database was used to study the relationship of TOP2A expression with the progression and prognosis of LUAD. For a further elucidation of the value of TOP2A in LUAD, the effect of TOP2A knockout on cell viability and related protein expression of LUAD cell line A549 in vitro was investigated by using RNA interference, MTT, flow cytometry, RT-PCR, and western blot analysis. RESULTS According to the results of database analysis, TOP2A expression in LUAD was higher than that in normal lung tissues. There was a strong correlation of TOP2A expression with clinicopathological and epidemiological parameters of LUAD. The survival rate of LUAD patients with high TOP2A expression was lower than that of patients with low expression (P&lt;0.001). The expression of TOP2A in A549 cells was higher than that in Beas-2B cells. After decreased expression of TOP2A in A549 cells, the proliferation of A549 cells was downregulated and the apoptosis rate was increased. It was further verified that TOP2A low expression exerts a role in LUAD through activation of the ERK/JNK/p-P38/CHOP signaling pathway. CONCLUSIONS The findings from this study showed that TOP2A expression was upregulated in a human lung adenocarcinoma cell line, and this finding was supported by bioinformatics analysis. Further studies are required to determine whether TOP2A expression is a prognostic biomarker and potential therapeutic target in patients with lung adenocarcinoma.</description><identifier>ISSN: 1643-3750</identifier><identifier>ISSN: 1234-1010</identifier><identifier>EISSN: 1643-3750</identifier><identifier>DOI: 10.12659/MSM.929120</identifier><identifier>PMID: 33361736</identifier><language>eng</language><publisher>United States: International Scientific Literature, Inc</publisher><subject>A549 Cells ; Adenocarcinoma of Lung - genetics ; Adenocarcinoma of Lung - pathology ; Database Analysis ; DNA Topoisomerases, Type II - genetics ; DNA Topoisomerases, Type II - metabolism ; Down-Regulation - genetics ; Gene Expression Regulation, Neoplastic ; Genetic Association Studies ; Humans ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; MAP Kinase Signaling System - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Treatment Outcome</subject><ispartof>Medical science monitor, 2020-12, Vol.26, p.e929120-e929120</ispartof><rights>Med Sci Monit, 2020 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-7717360f92f0568d905df7284b1db777e9fe5589fe37c66b26bcb5bea8eb7ab03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774312/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774312/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33361736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Xiaomei</creatorcontrib><creatorcontrib>Xue, Zhiwen</creatorcontrib><creatorcontrib>Lv, Jianning</creatorcontrib><creatorcontrib>Wang, Heidou</creatorcontrib><title>Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes</title><title>Medical science monitor</title><addtitle>Med Sci Monit</addtitle><description>BACKGROUND This study was carried out to analyze TOP2A expression in lung adenocarcinoma (LUAD) and to assess its value in clinical diagnosis and prognosis. MATERIAL AND METHODS The Cancer Genome Atlas (TCGA) database was used to study the relationship of TOP2A expression with the progression and prognosis of LUAD. For a further elucidation of the value of TOP2A in LUAD, the effect of TOP2A knockout on cell viability and related protein expression of LUAD cell line A549 in vitro was investigated by using RNA interference, MTT, flow cytometry, RT-PCR, and western blot analysis. RESULTS According to the results of database analysis, TOP2A expression in LUAD was higher than that in normal lung tissues. There was a strong correlation of TOP2A expression with clinicopathological and epidemiological parameters of LUAD. The survival rate of LUAD patients with high TOP2A expression was lower than that of patients with low expression (P&lt;0.001). The expression of TOP2A in A549 cells was higher than that in Beas-2B cells. After decreased expression of TOP2A in A549 cells, the proliferation of A549 cells was downregulated and the apoptosis rate was increased. It was further verified that TOP2A low expression exerts a role in LUAD through activation of the ERK/JNK/p-P38/CHOP signaling pathway. CONCLUSIONS The findings from this study showed that TOP2A expression was upregulated in a human lung adenocarcinoma cell line, and this finding was supported by bioinformatics analysis. Further studies are required to determine whether TOP2A expression is a prognostic biomarker and potential therapeutic target in patients with lung adenocarcinoma.</description><subject>A549 Cells</subject><subject>Adenocarcinoma of Lung - genetics</subject><subject>Adenocarcinoma of Lung - pathology</subject><subject>Database Analysis</subject><subject>DNA Topoisomerases, Type II - genetics</subject><subject>DNA Topoisomerases, Type II - metabolism</subject><subject>Down-Regulation - genetics</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic Association Studies</subject><subject>Humans</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>MAP Kinase Signaling System - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Treatment Outcome</subject><issn>1643-3750</issn><issn>1234-1010</issn><issn>1643-3750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpVkd1L5DAUxcPisn7sPvkueVRk3DRpk_ZFKIO6AyMj7OxzSNJbJ9ImNWn9AP_4jY4rbh6SAzn87rkchA4zcpZRXlQ_r39fn1W0yij5gvYynrMZEwXZ-aR30X6Md4TQkpPiG9pljPFMML6HXi6ehgAxWu-wb_G4Abz2g7fR9xBUBLxY4LobNgofr1c3tD7BV-AAW4eXk7vFdQPOGxWMdb5XeA5dF7FyzRuojtEbq8ZX9qMdN_gmaXAjXk2jSfz4HX1tVRfhx_t7gP5cXqznv2bL1dViXi9nJqdsnAnxmpW0FW1JwcumIkXTClrmOmu0EAKqFoqiTDcThnNNuTa60KBK0EJpwg7Q-ZY7TLqHxqQMQXVyCLZX4Vl6ZeX_P85u5K1_kAmes4wmwPE7IPj7CeIoextNWlY58FOUNBeMVenwZD3dWk3wMQZoP8ZkRL71JVNfcttXch99Tvbh_VcQ-wvU_5HX</recordid><startdate>20201227</startdate><enddate>20201227</enddate><creator>Du, Xiaomei</creator><creator>Xue, Zhiwen</creator><creator>Lv, Jianning</creator><creator>Wang, Heidou</creator><general>International Scientific Literature, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201227</creationdate><title>Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes</title><author>Du, Xiaomei ; Xue, Zhiwen ; Lv, Jianning ; Wang, Heidou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-7717360f92f0568d905df7284b1db777e9fe5589fe37c66b26bcb5bea8eb7ab03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>A549 Cells</topic><topic>Adenocarcinoma of Lung - genetics</topic><topic>Adenocarcinoma of Lung - pathology</topic><topic>Database Analysis</topic><topic>DNA Topoisomerases, Type II - genetics</topic><topic>DNA Topoisomerases, Type II - metabolism</topic><topic>Down-Regulation - genetics</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic Association Studies</topic><topic>Humans</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>MAP Kinase Signaling System - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Treatment Outcome</topic><toplevel>online_resources</toplevel><creatorcontrib>Du, Xiaomei</creatorcontrib><creatorcontrib>Xue, Zhiwen</creatorcontrib><creatorcontrib>Lv, Jianning</creatorcontrib><creatorcontrib>Wang, Heidou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical science monitor</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Xiaomei</au><au>Xue, Zhiwen</au><au>Lv, Jianning</au><au>Wang, Heidou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes</atitle><jtitle>Medical science monitor</jtitle><addtitle>Med Sci Monit</addtitle><date>2020-12-27</date><risdate>2020</risdate><volume>26</volume><spage>e929120</spage><epage>e929120</epage><pages>e929120-e929120</pages><issn>1643-3750</issn><issn>1234-1010</issn><eissn>1643-3750</eissn><abstract>BACKGROUND This study was carried out to analyze TOP2A expression in lung adenocarcinoma (LUAD) and to assess its value in clinical diagnosis and prognosis. MATERIAL AND METHODS The Cancer Genome Atlas (TCGA) database was used to study the relationship of TOP2A expression with the progression and prognosis of LUAD. For a further elucidation of the value of TOP2A in LUAD, the effect of TOP2A knockout on cell viability and related protein expression of LUAD cell line A549 in vitro was investigated by using RNA interference, MTT, flow cytometry, RT-PCR, and western blot analysis. RESULTS According to the results of database analysis, TOP2A expression in LUAD was higher than that in normal lung tissues. There was a strong correlation of TOP2A expression with clinicopathological and epidemiological parameters of LUAD. The survival rate of LUAD patients with high TOP2A expression was lower than that of patients with low expression (P&lt;0.001). The expression of TOP2A in A549 cells was higher than that in Beas-2B cells. After decreased expression of TOP2A in A549 cells, the proliferation of A549 cells was downregulated and the apoptosis rate was increased. It was further verified that TOP2A low expression exerts a role in LUAD through activation of the ERK/JNK/p-P38/CHOP signaling pathway. CONCLUSIONS The findings from this study showed that TOP2A expression was upregulated in a human lung adenocarcinoma cell line, and this finding was supported by bioinformatics analysis. Further studies are required to determine whether TOP2A expression is a prognostic biomarker and potential therapeutic target in patients with lung adenocarcinoma.</abstract><cop>United States</cop><pub>International Scientific Literature, Inc</pub><pmid>33361736</pmid><doi>10.12659/MSM.929120</doi><oa>free_for_read</oa></addata></record>
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subjects A549 Cells
Adenocarcinoma of Lung - genetics
Adenocarcinoma of Lung - pathology
Database Analysis
DNA Topoisomerases, Type II - genetics
DNA Topoisomerases, Type II - metabolism
Down-Regulation - genetics
Gene Expression Regulation, Neoplastic
Genetic Association Studies
Humans
Lung Neoplasms - genetics
Lung Neoplasms - pathology
MAP Kinase Signaling System - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Treatment Outcome
title Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes
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