Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes

Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. gra...

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Bibliographic Details
Published in:Phytopathology 2017-12, Vol.107 (12), p.1496-1506
Main Authors: Aoun, Meriem, Kolmer, James A, Rouse, Matthew N, Chao, Shiaoman, Bulbula, Worku Denbel, Elias, Elias M, Acevedo, Maricelis
Format: Article
Language:English
Subjects:
Basidiomycota - physiology
Chromosome Mapping
Chromosomes, Plant - genetics
Disease Resistance - genetics
Genetic Loci - genetics
Genetics and Resistance
Genotype
Phenotype
Plant Diseases - immunology
Plant Diseases - microbiology
Plant Leaves - genetics
Plant Leaves - immunology
Plant Leaves - microbiology
Plant Stems - genetics
Plant Stems - immunology
Plant Stems - microbiology
Polymorphism, Single Nucleotide - genetics
Seedlings - genetics
Seedlings - immunology
Seedlings - microbiology
Triticum - genetics
Triticum - immunology
Triticum - microbiology
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Summary:Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-12-16-0444-R