Chemopreventive and anticancer activities of indomethacin and vitamin D combination on colorectal cancer induced by 1,2-dimethylhydrazine in rats

• Published in: Biomedical reports 2021-02, Vol.14 (2), p.27, Article 27
• Main Authors: Okda, Tarek M, Abd-Elghaffar, Sary K, Katary, Mohamed A, Abd-Alhaseeb, Mohammad M
• Format: Article
• Language: English
• Subjects: 1,2-Dimethylhydrazine; Alfacalcidol; Anesthesia; Animals; Anticancer properties; Antigens; Antioxidants; Arthritis; Calcifediol; Calciferol; Cancer; Cancer therapies; Carcinoembryonic antigen; Carcinogens; Caspase-3; CEA (Oncology); Cell adhesion & migration; Colon; Colon cancer; Colorectal cancer; Colorectal carcinoma; Dimethylhydrazines; Ethylenediaminetetraacetic acid; Growth factors; Health aspects; Homogenization; Indomethacin; Inflammation; Inflammatory diseases; Mortality; Nonsteroidal anti-inflammatory drugs; Oncology, Experimental; Oral administration; Platelet-derived growth factor; Prevention; Proteins; Tumors; Vitamin D
• ISSN: 2049-9434; 2049-9442
• DOI: 10.3892/br.2020.1403

Several studies have revealed that the combination of indomethacin, a nonsteroidal anti-inflammatory drug (NSAID), and vitamin D reduces the risk of common types of cancers. Nonetheless, research on the deal concentrations used to test the impact of vitamin D on colon cancer is deficient. Along these lines, the aim of the present study was to evaluate the possible role of indomethacin and vitamin D as a preventative as well as a therapeutic operator for colon cancer growth induced by dimethylhydrazine (DMH) in male Albino rats. Fifty male albino rats were utilized in this examination; five groups were assigned from the animals (10 animals each): i) control group considered healthy animals; ii) carcinogen group that received DMH only; iii) prophylactic group; iv) vitamin D and indomethacin-treated group; and v) 5-flurouracil (5-FU) group. Western blot technique was used to determine the expression of carcinoembryonic antigen (CEA) and platelet-derived growth factor (PDGF). Overexpression of CEA and PDGF was noted in the carcinogenic group, while expression of CEA and PDGF in the prophylactic, vitamin D and indomethacin and 5-FU groups were markedly reduced. There was a likewise decline in tissue caspase-3 activity and antioxidant parameters in the carcinogenic group, while, there was an increase in these markers in the 5-FU group as well as the prophylactic and vitamin D and indomethacin groups. The combination of vitamin D and indomethacin markedly reduced the incidence and severity of colon cancer. The molecular, biochemical and histopathological analysis related with the oral administration of vitamin D and indomethacin display its capacity to limit the frequency of colorectal cancer.