Toxicity evaluation and biodegradation of phenanthrene by laccase from Trametes polyzona PBURU 12

The newly isolated Trametes polyzona PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by Trametes polyzona PBURU 12 was able to degrade phenan...

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Bibliographic Details
Published in:3 Biotech 2021, Vol.11 (1), p.32, Article 32
Main Authors: Wulandari, Retno, Lotrakul, Pongtharin, Punnapayak, Hunsa, Amirta, Rudianto, Kim, Seung Wook, Prasongsuk, Sehanat
Format: Article
Language:English
Subjects:
Agriculture
Ames test
Biodegradation
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Chemistry
Chemistry and Materials Science
E coli
Genotoxicity
Laccase
Metabolites
Microorganisms
Original
Original Article
Phenanthrene
Phytotoxicity
Polycyclic aromatic hydrocarbons
Stem Cells
Toxicity
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Summary:The newly isolated Trametes polyzona PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by Trametes polyzona PBURU 12 was able to degrade phenanthrene by up to 98% within 24 h. The degradation metabolites showed the absence of toxic compounds. Microbial viability tests using E. coli and B. subtilis revealed that the treated phenanthrene was less toxic than untreated phenanthrene. Phytotoxicity and genotoxicity tests, using Vigna radiata and Allium cepa , indicated that the treated phenanthrene was less toxic to the plants. No mutagenic activity was found in the Ames test. The crude laccase from Trametes polyzona PBURU 12 was demonstrated as a potential tool for the biodegradation of PAHs (phenanthrene), with low toxic effects after the degradation.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-020-02556-z