Complete Chloroplast Genome Sequencing and Phylogenetic Analysis of Two Dracocephalum Plants

Dracocephalum tanguticum and Dracocephalum moldavica are important herbs from Lamiaceae and have great medicinal value. We used the Illumina sequencing technology to sequence the complete chloroplast genome of D. tanguticum and D. moldavica and then conducted de novo assembly. The two chloroplast ge...

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Bibliographic Details
Published in:BioMed research international 2020, Vol.2020 (2020), p.1-9
Main Authors: Yao, Min, Ma, Xiaojing, Liao, Zhihua, Lan, Xiaozhong, Liu, Jiang, Hao, Peiyu, Su, Yanjie, Quan, Hong, Zhao, Kaihui, Xu, Yuanjiang, Zhao, Fangyu, Yao, Junjun, Yu, Benxia
Format: Article
Language:English
Subjects:
Base Composition
Chloroplasts
Chloroplasts - metabolism
Cytochrome
DNA sequencing
Dracocephalum
Dracocephalum moldavica
Evolution
Gene sequencing
Genes
Genes, Plant
Genetic aspects
Genetic engineering
Genome, Chloroplast
Genome, Plant
Genomes
High-Throughput Nucleotide Sequencing
Inverted repeat
Lamiaceae
Lamiaceae - genetics
Methods
Microsatellite Repeats
Mutation
Mutation hot spots
Nucleotide sequence
Nucleotide sequencing
Phylogenetics
Phylogeny
Plants, Medicinal - genetics
Proteins
RNA polymerase
RNA, Transfer - metabolism
Rps2 gene
rRNA
Sequence Analysis, DNA
tRNA
Whole Genome Sequencing
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Summary:Dracocephalum tanguticum and Dracocephalum moldavica are important herbs from Lamiaceae and have great medicinal value. We used the Illumina sequencing technology to sequence the complete chloroplast genome of D. tanguticum and D. moldavica and then conducted de novo assembly. The two chloroplast genomes have a typical quadripartite structure, with the gene’s lengths of 82,221 bp and 81,450 bp, large single-copy region’s (LSC) lengths of 82,221 bp and 81,450 bp, and small single-copy region’s (SSC) lengths of 17,363 bp and 17,066 bp, inverted repeat region’s (IR) lengths of 51,370 bp and 51,352 bp, respectively. The GC content of the two chloroplast genomes was 37.80% and 37.83%, respectively. The chloroplast genomes of the two plants encode 133 and 132 genes, respectively, among which there are 88 and 87 protein-coding genes, respectively, as well as 37 tRNA genes and 8 rRNA genes. Among them, the rps2 gene is unique to D. tanguticum, which is not found in D. moldavica. Through SSR analysis, we also found 6 mutation hotspot regions, which can be used as molecular markers for taxonomic studies. Phylogenetic analysis showed that Dracocephalum was more closely related to Mentha.
ISSN:2314-6133
2314-6141
DOI:10.1155/2020/4374801