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Persistence of Markers of Chloroquine Resistance in Plasmodium falciparum in Bangladesh
The control of malaria, in terms of drug resistance, remains a significant global challenge, with Bangladesh, a malaria-endemic country, being no exception. The aim of this study was to explore antimalarial resistance in Bangladesh by molecular analysis of chloroquine resistance transporter ( ) and...
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| Published in: | The American journal of tropical medicine and hygiene 2021-01, Vol.104 (1), p.276-282 |
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| creator | Johora, Fatema Tuj Elahi, Rubayet Nima, Maisha Khair Hossain, Mohammad Sharif Rashid, Humaira Kibria, Mohammad Golam Mohon, Abu Naser Khan, Wasif A Haque, Rashidul Alam, Mohammad Shafiul |
| description | The control of malaria, in terms of drug resistance, remains a significant global challenge, with Bangladesh, a malaria-endemic country, being no exception. The aim of this study was to explore antimalarial resistance in Bangladesh by molecular analysis of
chloroquine resistance transporter (
) and
multidrug resistance transporter 1 (
) genetic markers of
.
. Samples were obtained from uncomplicated malaria patients between 2009 and 2014 from six malaria-endemic districts. Based on parasite transmission intensity, the endemic districts were divided into high-transmission (Chittagong Hill Tracts [CHT]) and low-transmission (non-CHT) regions. Falciparum malaria-positive isolates were genotyped for K76T of the
gene, and N86Y and Y184F of the
gene: in total, 262
clinical isolates were analyzed. In CHT areas, the prevalence of polymorphisms was 70.6% for 76T, 14.4% for 86Y, and 7.8% for 184F. In non-CHT areas, 76T and 86Y mutations were found in 78.0% and 19.5% of the samples, respectively, whereas no 184F mutations were observed. We compared our data with previous similar molecular observations, which shows a significant decrease in
76T mutation prevalence. No
amplification was observed in any of the samples suggesting an unaltered susceptibility to amino alcohol drugs such as mefloquine and lumefantrine. This study provides an updated assessment of the current status of
and
gene mutations in Bangladesh, and suggests there is persistent high prevalence of markers of resistance to aminoquinoline drugs. |
| doi_str_mv | 10.4269/AJTMH.20-0415 |
| format | article |
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chloroquine resistance transporter (
) and
multidrug resistance transporter 1 (
) genetic markers of
.
. Samples were obtained from uncomplicated malaria patients between 2009 and 2014 from six malaria-endemic districts. Based on parasite transmission intensity, the endemic districts were divided into high-transmission (Chittagong Hill Tracts [CHT]) and low-transmission (non-CHT) regions. Falciparum malaria-positive isolates were genotyped for K76T of the
gene, and N86Y and Y184F of the
gene: in total, 262
clinical isolates were analyzed. In CHT areas, the prevalence of polymorphisms was 70.6% for 76T, 14.4% for 86Y, and 7.8% for 184F. In non-CHT areas, 76T and 86Y mutations were found in 78.0% and 19.5% of the samples, respectively, whereas no 184F mutations were observed. We compared our data with previous similar molecular observations, which shows a significant decrease in
76T mutation prevalence. No
amplification was observed in any of the samples suggesting an unaltered susceptibility to amino alcohol drugs such as mefloquine and lumefantrine. This study provides an updated assessment of the current status of
and
gene mutations in Bangladesh, and suggests there is persistent high prevalence of markers of resistance to aminoquinoline drugs.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/AJTMH.20-0415</identifier><identifier>PMID: 33146120</identifier><language>eng</language><publisher>United States: Institute of Tropical Medicine</publisher><subject>Antimalarials - pharmacology ; Bangladesh - epidemiology ; Chloroquine - pharmacology ; Drug Resistance ; Erythrocytes ; Genetic Markers ; Genotype ; Humans ; Malaria ; Malaria, Falciparum - epidemiology ; Malaria, Falciparum - parasitology ; Membrane Transport Proteins - genetics ; Multidrug Resistance-Associated Proteins - genetics ; Mutation ; Plasmodium falciparum - drug effects ; Plasmodium falciparum - genetics ; Polymorphism, Genetic ; Protozoan Proteins - genetics ; Time Factors</subject><ispartof>The American journal of tropical medicine and hygiene, 2021-01, Vol.104 (1), p.276-282</ispartof><rights>Copyright Institute of Tropical Medicine Jan 2021</rights><rights>The American Society of Tropical Medicine and Hygiene 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-455103ad34cac8dcacb6fd23824d1f13261b5b80da08b4bce7376f88fcd7acd33</citedby><cites>FETCH-LOGICAL-c415t-455103ad34cac8dcacb6fd23824d1f13261b5b80da08b4bce7376f88fcd7acd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790087/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790087/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33146120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johora, Fatema Tuj</creatorcontrib><creatorcontrib>Elahi, Rubayet</creatorcontrib><creatorcontrib>Nima, Maisha Khair</creatorcontrib><creatorcontrib>Hossain, Mohammad Sharif</creatorcontrib><creatorcontrib>Rashid, Humaira</creatorcontrib><creatorcontrib>Kibria, Mohammad Golam</creatorcontrib><creatorcontrib>Mohon, Abu Naser</creatorcontrib><creatorcontrib>Khan, Wasif A</creatorcontrib><creatorcontrib>Haque, Rashidul</creatorcontrib><creatorcontrib>Alam, Mohammad Shafiul</creatorcontrib><title>Persistence of Markers of Chloroquine Resistance in Plasmodium falciparum in Bangladesh</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>The control of malaria, in terms of drug resistance, remains a significant global challenge, with Bangladesh, a malaria-endemic country, being no exception. The aim of this study was to explore antimalarial resistance in Bangladesh by molecular analysis of
chloroquine resistance transporter (
) and
multidrug resistance transporter 1 (
) genetic markers of
.
. Samples were obtained from uncomplicated malaria patients between 2009 and 2014 from six malaria-endemic districts. Based on parasite transmission intensity, the endemic districts were divided into high-transmission (Chittagong Hill Tracts [CHT]) and low-transmission (non-CHT) regions. Falciparum malaria-positive isolates were genotyped for K76T of the
gene, and N86Y and Y184F of the
gene: in total, 262
clinical isolates were analyzed. In CHT areas, the prevalence of polymorphisms was 70.6% for 76T, 14.4% for 86Y, and 7.8% for 184F. In non-CHT areas, 76T and 86Y mutations were found in 78.0% and 19.5% of the samples, respectively, whereas no 184F mutations were observed. We compared our data with previous similar molecular observations, which shows a significant decrease in
76T mutation prevalence. No
amplification was observed in any of the samples suggesting an unaltered susceptibility to amino alcohol drugs such as mefloquine and lumefantrine. This study provides an updated assessment of the current status of
and
gene mutations in Bangladesh, and suggests there is persistent high prevalence of markers of resistance to aminoquinoline drugs.</description><subject>Antimalarials - pharmacology</subject><subject>Bangladesh - epidemiology</subject><subject>Chloroquine - pharmacology</subject><subject>Drug Resistance</subject><subject>Erythrocytes</subject><subject>Genetic Markers</subject><subject>Genotype</subject><subject>Humans</subject><subject>Malaria</subject><subject>Malaria, Falciparum - epidemiology</subject><subject>Malaria, Falciparum - parasitology</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Multidrug Resistance-Associated Proteins - genetics</subject><subject>Mutation</subject><subject>Plasmodium falciparum - drug effects</subject><subject>Plasmodium falciparum - genetics</subject><subject>Polymorphism, Genetic</subject><subject>Protozoan Proteins - genetics</subject><subject>Time Factors</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc1LHTEUxUOx1Kftstsy4MbNPG8-JslsBH1oVRSlWLoMmSTjy3Nm8kxmCv3vm0Er1k1yuefH4R4OQl8xLBnh9dHJ1f3NxZJACQxXH9ACM8FLzFm1gxYAQMqaU7GL9lLaAGBJMP6EdinFjGMCC_TrzsXk0-gG44rQFjc6PubNPK7WXYjhafKDK364GdIz5IfirtOpD9ZPfdHqzvitjnnMwqkeHjptXVp_Rh-zlNyXl38f_Tw_u19dlNe33y9XJ9elyeeOJasqDFRbyow20uan4a0lVBJmcYsp4bipGglWg2xYY5yggrdStsYKbSyl--j42Xc7Nb2zxg1j1J3aRt_r-EcF7dX_yuDX6iH8VkLUAFJkg8MXgzmrS6PqfTKu6_TgwpQUYZXgEtdCZvTgHboJUxxyPEUqIlldC6gzVT5TJoaUomtfj8Gg5sqU3oz9WhFQc2WZ__Y2wSv9ryP6FzQplBQ</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Johora, Fatema Tuj</creator><creator>Elahi, Rubayet</creator><creator>Nima, Maisha Khair</creator><creator>Hossain, Mohammad Sharif</creator><creator>Rashid, Humaira</creator><creator>Kibria, Mohammad Golam</creator><creator>Mohon, Abu Naser</creator><creator>Khan, Wasif A</creator><creator>Haque, Rashidul</creator><creator>Alam, Mohammad Shafiul</creator><general>Institute of Tropical Medicine</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210101</creationdate><title>Persistence of Markers of Chloroquine Resistance in Plasmodium falciparum in Bangladesh</title><author>Johora, Fatema Tuj ; Elahi, Rubayet ; Nima, Maisha Khair ; Hossain, Mohammad Sharif ; Rashid, Humaira ; Kibria, Mohammad Golam ; Mohon, Abu Naser ; Khan, Wasif A ; Haque, Rashidul ; Alam, Mohammad Shafiul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-455103ad34cac8dcacb6fd23824d1f13261b5b80da08b4bce7376f88fcd7acd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antimalarials - pharmacology</topic><topic>Bangladesh - epidemiology</topic><topic>Chloroquine - pharmacology</topic><topic>Drug Resistance</topic><topic>Erythrocytes</topic><topic>Genetic Markers</topic><topic>Genotype</topic><topic>Humans</topic><topic>Malaria</topic><topic>Malaria, Falciparum - epidemiology</topic><topic>Malaria, Falciparum - parasitology</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Multidrug Resistance-Associated Proteins - genetics</topic><topic>Mutation</topic><topic>Plasmodium falciparum - drug effects</topic><topic>Plasmodium falciparum - genetics</topic><topic>Polymorphism, Genetic</topic><topic>Protozoan Proteins - genetics</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johora, Fatema Tuj</creatorcontrib><creatorcontrib>Elahi, Rubayet</creatorcontrib><creatorcontrib>Nima, Maisha Khair</creatorcontrib><creatorcontrib>Hossain, Mohammad Sharif</creatorcontrib><creatorcontrib>Rashid, Humaira</creatorcontrib><creatorcontrib>Kibria, Mohammad Golam</creatorcontrib><creatorcontrib>Mohon, Abu Naser</creatorcontrib><creatorcontrib>Khan, Wasif A</creatorcontrib><creatorcontrib>Haque, Rashidul</creatorcontrib><creatorcontrib>Alam, Mohammad Shafiul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johora, Fatema Tuj</au><au>Elahi, Rubayet</au><au>Nima, Maisha Khair</au><au>Hossain, Mohammad Sharif</au><au>Rashid, Humaira</au><au>Kibria, Mohammad Golam</au><au>Mohon, Abu Naser</au><au>Khan, Wasif A</au><au>Haque, Rashidul</au><au>Alam, Mohammad Shafiul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Persistence of Markers of Chloroquine Resistance in Plasmodium falciparum in Bangladesh</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>104</volume><issue>1</issue><spage>276</spage><epage>282</epage><pages>276-282</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><abstract>The control of malaria, in terms of drug resistance, remains a significant global challenge, with Bangladesh, a malaria-endemic country, being no exception. The aim of this study was to explore antimalarial resistance in Bangladesh by molecular analysis of
chloroquine resistance transporter (
) and
multidrug resistance transporter 1 (
) genetic markers of
.
. Samples were obtained from uncomplicated malaria patients between 2009 and 2014 from six malaria-endemic districts. Based on parasite transmission intensity, the endemic districts were divided into high-transmission (Chittagong Hill Tracts [CHT]) and low-transmission (non-CHT) regions. Falciparum malaria-positive isolates were genotyped for K76T of the
gene, and N86Y and Y184F of the
gene: in total, 262
clinical isolates were analyzed. In CHT areas, the prevalence of polymorphisms was 70.6% for 76T, 14.4% for 86Y, and 7.8% for 184F. In non-CHT areas, 76T and 86Y mutations were found in 78.0% and 19.5% of the samples, respectively, whereas no 184F mutations were observed. We compared our data with previous similar molecular observations, which shows a significant decrease in
76T mutation prevalence. No
amplification was observed in any of the samples suggesting an unaltered susceptibility to amino alcohol drugs such as mefloquine and lumefantrine. This study provides an updated assessment of the current status of
and
gene mutations in Bangladesh, and suggests there is persistent high prevalence of markers of resistance to aminoquinoline drugs.</abstract><cop>United States</cop><pub>Institute of Tropical Medicine</pub><pmid>33146120</pmid><doi>10.4269/AJTMH.20-0415</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The American journal of tropical medicine and hygiene, 2021-01, Vol.104 (1), p.276-282 |
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| language | eng |
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| source | PubMed Central Free |
| subjects | Antimalarials - pharmacology Bangladesh - epidemiology Chloroquine - pharmacology Drug Resistance Erythrocytes Genetic Markers Genotype Humans Malaria Malaria, Falciparum - epidemiology Malaria, Falciparum - parasitology Membrane Transport Proteins - genetics Multidrug Resistance-Associated Proteins - genetics Mutation Plasmodium falciparum - drug effects Plasmodium falciparum - genetics Polymorphism, Genetic Protozoan Proteins - genetics Time Factors |
| title | Persistence of Markers of Chloroquine Resistance in Plasmodium falciparum in Bangladesh |
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