Ultrastructure of Felis catus whole fetus (Fcwf-4) cell culture following infection with feline coronavirus

Feline coronavirus (FCoV) consists of two biotypes based on their growth in cell culture and their antigenicity. Infections with FCoV are highly prevalent in the cat population worldwide. In this study, Felis catus whole fetus (Fcwf-4)cell culture was infected with FCoV UPM11C/08. Virus multiplicati...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Electron Microscopy 2011-08, Vol.60 (4), p.275-282
Main Authors: Alazawy, Amer, Arshad, Siti-Suri, Bejo, Mohd-Hair, Omar, Abdul-Rahman, Tengku Ibrahim, Tengku-Azmi, Sharif, Saeed, Bande, Faruku, Awang-Isa, Kamarudin
Format: Article
Language:English
Subjects:
Animals
Antigenicity
Biological: Full-length
Cats
Cell culture
Cell Line
Cells, Cultured - ultrastructure
Cells, Cultured - virology
Coronavirus, Feline - isolation & purification
Coronavirus, Feline - pathogenicity
Coronavirus, Feline - ultrastructure
Cytoplasm - ultrastructure
Cytoplasm - virology
Feline coronavirus
Feline Infectious Peritonitis - virology
Fetuses
Microscopy, Electron, Transmission
Morphology
Vacuoles - ultrastructure
Vacuoles - virology
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Feline coronavirus (FCoV) consists of two biotypes based on their growth in cell culture and their antigenicity. Infections with FCoV are highly prevalent in the cat population worldwide. In this study, Felis catus whole fetus (Fcwf-4)cell culture was infected with FCoV UPM11C/08. Virus multiplication in cell culture was monitored and examined under the transmission electron microscope. The virus particles revealed the characteristic morphology of feline FCoV represented by envelope viruses surrounded by peplomers. Virus attachment and entry into the cell occurred 15 h post-infection (pi), and the myriad of virus particles were observed both extracellularly and intracellularly after 48 h pi. Thereafter, intracellular virus particles were observed to be present in vacuoles or present freely in the cytoplasm.
ISSN:0022-0744
1477-9986
2050-5701
DOI:10.1093/jmicro/dfr031