Bacterial and eukaryotic microbial communities in urban water systems profiled via Illumina MiSeq platform

Microbial communities from a lake and river flowing through a highly dense urbanized township in Malaysia were profiled by sequencing amplicons of the 16S V3–V4 and 18S V9 hypervariable rRNA gene regions via Illumina MiSeq. Results revealed that Proteobacteria, Bacteroidetes, Actinobacteria and Firm...

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Bibliographic Details
Published in:3 Biotech 2021-02, Vol.11 (2), p.40-40, Article 40
Main Authors: Ting, Adeline Su Yien, Zoqratt, Muhammad Zarul Hanifah Md, Tan, Hock Siew, Hermawan, Andreas Aditya, Talei, Amin, Khu, Soon Thiam
Format: Article
Language:English
Subjects:
Abundance
Agriculture
Aquatic communities
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Chemistry
Chemistry and Materials Science
Cyanobacteria
Human influences
Land use
Microbiomes
Microorganisms
Most probable number
Opportunist infection
Original
Original Article
Pathogens
Physicochemical properties
rRNA 18S
Stem Cells
Urbanization
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Summary:Microbial communities from a lake and river flowing through a highly dense urbanized township in Malaysia were profiled by sequencing amplicons of the 16S V3–V4 and 18S V9 hypervariable rRNA gene regions via Illumina MiSeq. Results revealed that Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the dominant prokaryotic phyla; whereas, eukaryotic communities were predominantly of the SAR clade and Opisthokonta. The abundance of Pseudomonas and Flavobacterium in all sites suggested the possible presence of pathogens in the urban water systems, supported by the most probable number (MPN) values of more than 1600 per 100 mL. Urbanization could have impacted the microbial communities as transient communities (clinical, water-borne and opportunistic pathogens) coexisted with common indigenous aquatic communities (Cyanobacteria). It was concluded that in urban water systems, microbial communities vary in their abundance of microbial phyla detected along the water systems. The influences of urban land use and anthropogenic activities influenced the physicochemical properties and the microbial dynamics in the water systems.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-020-02617-3