Cancer Signaling Transcriptome Is Upregulated in Type 2 Diabetes Mellitus

We aimed to explore the differences in the whole transcriptome of peripheral blood mononuclear cells between elderly individuals with and without type 2 diabetes (T2D). We conducted a microarray-based transcriptome analysis of 19 individuals with T2D and 15 without. Differentially expressed genes ac...

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Bibliographic Details
Published in:Journal of clinical medicine 2020-12, Vol.10 (1), p.85
Main Authors: Almanza-Aguilera, Enrique, Hernáez, Álvaro, Corella, Dolores, Sanllorente, Albert, Ros, Emilio, Portolés, Olga, Valussi, Julieta, Estruch, Ramon, Coltell, Oscar, Subirana, Isaac, Canudas, Silvia, Razquin, Cristina, Blanchart, Gemma, Nonell, Lara, Fitó, Montserrat, Castañer, Olga
Format: Article
Language:English
Subjects:
Cancer
Cardiovascular disease
Clinical medicine
Diabetes
Experiments
Gene expression
Genomes
Glucose
Insulin
Performance evaluation
Software
Statistics
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Summary:We aimed to explore the differences in the whole transcriptome of peripheral blood mononuclear cells between elderly individuals with and without type 2 diabetes (T2D). We conducted a microarray-based transcriptome analysis of 19 individuals with T2D and 15 without. Differentially expressed genes according to linear models were submitted to the Ingenuity Pathway Analysis system to conduct a functional enrichment analysis. We established that diseases, biological functions, and canonical signaling pathways were significantly associated with T2D patients when their logarithms of Benjamini-Hochberg-adjusted -value were >1.30 and their absolute z-scores were >2.0 (≥2.0 meant "upregulation" and ≤ -2.0 "downregulation"). Cancer signaling pathways were the most upregulated ones in T2D (z-score = 2.63, -log( -value) = 32.3; 88.5% ( = 906) of the total differentially expressed genes located in these pathways). In particular, integrin (z-score = 2.52, -log( -value) = 2.03) and paxillin (z-score = 2.33, -log( -value) = 1.46) signaling pathways were predicted to be upregulated, whereas the Rho guanosine diphosphate (Rho-GDP) dissociation inhibitor signaling pathway was predicted to be downregulated in T2D individuals (z-score = -2.14, -log( -value) = 2.41). Our results suggest that, at transcriptional expression level, elderly individuals with T2D present an increased activation of signaling pathways related to neoplastic processes, T-cell activation and migration, and inflammation.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm10010085