Effect of D614G Spike Variant on Immunoglobulin G, M, or A Spike Seroassay Performance

Abstract Emergence of a new spike protein variant (D614G) with increased infectivity has prompted many to analyze its role in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. There is concern regarding whether an individual exposed to one variant of a virus will have cross-...

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Bibliographic Details
Published in:The Journal of infectious diseases 2021-03, Vol.223 (5), p.802-804
Main Authors: Klumpp-Thomas, Carleen, Kalish, Heather, Hicks, Jennifer, Mehalko, Jennifer, Drew, Matthew, Memoli, Matthew J, Hall, Matthew D, Esposito, Dominic, Sadtler, Kaitlyn
Format: Article
Language:English
Subjects:
Antibodies, Viral - blood
Coronaviruses
COVID-19
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 - virology
Cross Reactions
Enzyme-Linked Immunosorbent Assay - methods
Humans
Humoral immunity
Immunoglobulin A - blood
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin M - blood
Immunoglobulins
Immunological memory
Infectivity
Major and Brief Reports
Mutation
Pandemics
SARS-CoV-2 - classification
SARS-CoV-2 - immunology
SARS-CoV-2 - pathogenicity
Severe acute respiratory syndrome coronavirus 2
Spike Glycoprotein, Coronavirus - genetics
Spike Glycoprotein, Coronavirus - immunology
Spike protein
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Summary:Abstract Emergence of a new spike protein variant (D614G) with increased infectivity has prompted many to analyze its role in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. There is concern regarding whether an individual exposed to one variant of a virus will have cross-reactive memory to the second variant. Accordingly, we analyzed the serologic reactivity of both variants, and we found that antibodies from 88 donors from a high-incidence population reacted toward both the original spike and the D614 spike variant. These data suggest that patients who are exposed to either variant have cross-responsive humoral immunity. This represents an important finding both for SARS-CoV-2 disease biology and for therapeutics. As the D614G spike variant emerged as the main severe acute respiratory syndrome coronavirus 2 variant in the United States, concerns arose regarding possible differences in antibody binding and serologic assay performance. We show that antibodies cross-react with both variants.
ISSN:0022-1899
1537-6613
1537-6613
DOI:10.1093/infdis/jiaa743