Left Stellate Ganglion Ablation Inhibits Ventricular Arrhythmias through Macrophage Regulation in Canines with Acute Ischemic Stroke

• Published in: International journal of medical sciences 2021, Vol.18 (4), p.891-901
• Main Authors: Wang, Youcheng, He, Shanqing, Xiong, Xiaoxing, Liu, Jia, Xie, Baojun, Yao, Yajun, Yin, Junkui, Zi, Liuliu, Wang, Xi, Tang, Yanhong, Zhao, Qingyan
• Format: Article
• Language: English
• Subjects: Ablation; Antibodies; Biotechnology; Blood pressure; Cardiac arrhythmia; Elk; Heart attacks; Laboratory animals; Magnetic resonance imaging; Ostomy; Research Paper; Stroke; Tumor necrosis factor-TNF; Veins & arteries
• ISSN: 1449-1907; 1449-1907
• DOI: 10.7150/ijms.50976

To investigate the potential mechanism of ventricular arrhythmias (VAs) after acute ischemic stroke and explore the effects of left stellate gangling (LSG) ablation on VAs induced by stroke in canines. Twenty canines were randomly divided into the sham-operated group (n=6), AS group (n=7) and SGA group (n=7). Cerebral ischemic model was established in the AS group and the SGA group by right acute middle cerebral artery occlusion (MCAO). LSG ablation was performed in the SGA group as soon as MCAO. After 3 days, atrial electrophysiology and neural activity were measured in vivo. The levels of norepinephrine (NE) in plasma and ventricle were detected by ELISA. The levels of monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-α (TNF-α) and NF-κB p65 in ventricle were detected by western blotting. The pro-inflammatory polarization of macrophages in ventricle was detected by immunofluorescence. Higher ventricular tachycardia (VT) inducibility and lower ventricular fibrillation threshold (VFT) were observed in the AS group compared with those in the sham-operated group, associated with higher LSG activity and NE levels, increased number of M1 macrophages and secretion of inflammatory cytokines in ventricle (all P